2024年12月18日 星期三
铁线莲组(铁线莲属)植物染色体核型分析
Chromosome karyotype analysis of Sect. Viticella DC. species (Clematis Linn.)
2024年 第33卷 第2期 页码[99-105]    下载全文[1.5MB]  
摘要

 采用常规压片法对铁线莲属(Clematis Linn.)铁线莲组(Sect. Viticella DC.)10种植物根尖进行制片,并进行染色体核型分析,同时基于核型似近系数对供试植物进行UPGMA聚类分析。结果表明:这些植物均为二倍体(2n=2x=16),并且,多数染色体为中部(m)着丝点染色体,部分染色体为近端部(st)着丝点染色体,少数染色体为近中部(sm)或端部(t)着丝点染色体;核型公式有10m+6st、10m+2sm+4st和10m+4st+2t 3种;吴兴铁线莲(C. huchouensis Tamura)的染色体相对长度范围(8.34~17.87)、最长染色体与最短染色体相对长度的比值(2.14)、平均臂比(3.15)均最大;吴兴铁线莲和意大利铁线莲(C. viticella Linn.)的核型为“2B”,其余种类的核型为“2A”;短柱铁线莲(C. cadmia Buch.-Ham. ex Wall.)的核型不对称系数最大(65.78%)、着丝点指数(31.88%)最小,而天台铁线莲〔C. patens ssp. tientaiensis (M. Y. Fang) W. T. Wang〕的核型不对称系数最小(61.28%)、着丝点指数(35.27%)最大。供试植物第1至第5对染色体均为中部着丝点染色体,第6至第8对染色体则为近中部、近端部或端部着丝点染色体。核型不对称性散布图显示:重瓣铁线莲(C. florida var. plena D. Don)、天台铁线莲、铁线莲(C. florida Thunb.)和转子莲(C. patens C. Morr. et Decne.)均位于左下角,说明这些植物的核型不对称性较低。在核型似近系数为0.948处,供试的10种植物分成2组。研究结果显示:供试铁线莲组10种植物中,重瓣铁线莲等4种植物处于相对原始的地位。

 

Abstract

 Chromosome slides of root tips of ten species of Sect. Viticella DC. in Clematis Linn. were prepared by using the conventional pressing method, and chromosome karyotype analysis was conducted, meanwhile, UPGMA cluster analysis was conducted for test plants based on karyotype resemblance-near coefficient. The results show that these plants are all diploid (2n=2x=16), moreover, most chromosomes are median (m) centromere chromosomes, some chromosomes are subterminal (st) centromere chromosomes, and a few chromosomes are submedian (sm) or terminal (t) centromere chromosomes; there are three types of karyotype formulas namely 10m+6st, 10m+2sm+4st, and 10m+4st+2t; the chromosome relative length range (8.34-17.87), ratio of relative length of the longest chromosome to the shortest chromosome (2.14), and average arm ratio (3.15) of C. huchouensis Tamura are all the largest; the karyotypes of C. huchouensis and C. viticella Linn. are “2B”, while those of the other species are  “2A”; the karyotype asymmetry coefficient (65.78%) of C. cadmia Buch.-Ham. ex Wall. is the largest and its centromeric index (31.88%) is the smallest, while the karyotype asymmetry coefficient (61.28%) of C. patens ssp. tientaiensis (M. Y. Fang) W. T. Wang. is the smallest and its centromeric index (35.27%) is the largest. The first to fifth pairs of chromosomes of test plants are all median centromere chromosomes, while the sixth to eighth pairs of chromosomes are submedian, subterminal, or terminal centromere chromosomes. The karyotype asymmetry scatter diagram shows that C. florida var. plena D. Don, C. patens ssp. tientaiensis, C. florida Thunb., and C. patens C. Morr. et Decne. are all located in the lower left corner, indicating that these plants have low karyotype asymmetry. At the karyotype resemblancenear coefficient of 0.948, the ten test species are divided into two groups. It is suggested that among the ten test species of Sect. Viticella, four species such as C. florida var. plena occupy a relative primitive position.

 

关键词铁线莲属; 铁线莲组; 染色体核型; 核型不对称系数; 核型似近系数
Key wordsClematis Linn.; Sect. Viticella DC.; chromosome karyotype; karyotype asymmetry coefficient; karyotype resemblance-near coefficient
作者陈铭健, 王淑安, 王鹏, 刘永东, 李素梅, 李林芳, 李亚
所在单位江苏省中国科学院植物研究所(南京中山植物园), 江苏 南京 210014
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下载次数1060
基金项目江苏省农业科技自主创新资金项目〔CX(20)3027〕; 江苏省植物资源研究与利用重点实验室开放基金项目(JSPKLB202203)