通过诱导残败花梗上的休眠芽萌发,以萌发的幼叶和去茎尖的茎段为外植体进行组织培养,建立了蝴蝶兰(Phalaenopsis amabilis Bl.)的无菌繁殖体系,并筛选出最佳培养基组成。诱导休眠芽萌发的最佳培养基为不加任何激素的MS0培养基;原球茎诱导的适宜培养基为MS+3.0mg·L^-16 BA+0.5mg·L^-1ZT+30mg·L^-1柠檬酸和MS+5.0mg·L^-16 BA+30mg·L^-1柠檬酸+30%椰乳(CM),其中茎段的诱导效果明显优于叶片,诱导率达95%;诱导无菌苗生根的最适培养基为1/4MS+1.0mg·L^-16 BA+0.1mg·L^-1NAA,生根率可达79%。

Flower peduncles of Phalaenopsis amabilis Bl. were cultured on MS₀ medium to induce seedlings, then leaflets and shoots without apex of the plantlet were cultured as explants to produce protocorm-like bodies in vitro. The suitable media inducing protocorm-like bodies were MS+ 3.0 mg·L^-1 6-BA+ 0.5 mg·L^-1 ZT+ 30 mg·L^-1 citric acid and MS+ 5.0 mg·L^-1 6-BA+ 30 mg·L^-1 citric acid+ 30% CM. Induction rate of shoots without apex was higher than that of leaves and it is up to 95%. The optimized medium for rooting was 1/4 MS+ 1.0 mg·L^-1 6-BA+ 0.1 mg·L^-1 NAA, the rooting rate reached to 79%.