用秋水仙素诱导何首乌(Polygonum multiflorumThunb.)同源多倍体的最佳条件为用0.2%秋水仙素溶液处理其试管苗幼嫩顶芽36 h,并接种于含2.0 mg·L^-16-BA和0.2 mg·L^-1IAA的MS启动培养基上。对诱导出的多倍体株系进行染色体计数分析,结果表明,诱导产生的多倍体均为四倍体,染色体基数2n=4x=44。生理指标测定结果表明,何首乌同源四倍体株系试管苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)和黄嘌呤氧化酶(APX)活性及叶绿素含量等指标均高于二倍体株系。 

Autopolyploids of Polygonum multiflorum Thunb. were induced with colchicine. The results indicated that optimal method for inducing autopolyploids was to immerse buds of P. multiflorum plantlet into 0. 2% colchicine solution for36 h, then let them grow in MS medium containing 2. 0 mg·L^-16-BAand 0. 2 mg·L^-1 IAA. Induced polyploids were identified by root-tips chromosome determination. It was showed that all of induced polyploids were tetraploids, and their chromosome number was 2n= 4x= 44. The results also showed that some physiological indices of autotetraploids, such as activities of SOD, POD and APX, and chlorophyll content, were much higher than that of diploids.