以南烛(Vaccinium bracteatum Thunb.)组培苗离体叶片为实验材料,研究了不同培养条件对其不定芽再生状况的影响并筛选出最适培养条件。结果表明:基本培养基类型、生长调节剂种类及质量浓度、琼脂和蔗糖质量浓度、外植体类型、外植体接种方式和暗培养时间均对南烛离体叶片不定芽的出芽时间和再生率、外植体干枯率以及不定芽的生长状况有明显影响。南烛离体叶片不定芽再生的最佳培养条件为:以中脉横切2 次的叶片为外植体,以近轴面面向培养基的方式接种;以1/2MS-1/2WPM 为基本培养基, 添加7. 5 g·L^-1 琼脂、25. 0 g·L^-1 蔗糖、0. 5 mg·L^-1 TDZ、5. 0 mg·L^-1 2ip 或4. 0 mg·L^-1 ZT,暗培养21 d 后转至光照度2 000 lx、光照时间16 h·d-1 的条件下培养。

Taking leaf in vitro of tissue culture seedling of Vaccinium bracteatum Thunb. as experimental materials, effects of different culture conditions on regeneration status of adventitious bud were studied, and the optimal culture condition was screened. The results show that basic medium type, type and mass concentration of growth regulator, mass concentrations of agar and sucrose, explant type, inoculating mode of explant and dark culture time have obvious effects on sprouting time and regeneration rate of adventitious bud, withering rate of explant and growth status of adventitious bud of leaf in vitro of V. bracteatum. The optimal culture condition for adventitious bud regeneration of leaf in vitro of V. bracteatum is as follow: taking leaf with midrib cut transversely for two times as explant, inoculating with its adaxial side facing on medium, taking 1/2MS-1/2WPM as basic medium and adding 7. 5 g·L^-1 agar, 25.0 g·L^-1 sucrose, 0. 5 mg·L^-1 TDZ, and 5. 0 mg·L^-12ip or 4. 0 mg·L^-1 ZT, dark culture for 21 d and then transferring to culture in the condition of illumination intensity 2 000 lx and illumination time 16 h·d^-1.