采用水培法,对0(对照)、12. 5、25、50、100、200 和400 mg·L^-1 槲皮黄素-3-α-阿拉伯糖苷(Q3A)和槲皮黄素-3-β-葡萄糖苷(Q3B)处理0 ~72 h 木麻黄(Casuarina equisetifolia Forst.)幼苗小枝活性氧代谢及保护酶活性的动态变化进行了研究。结果表明:随Q3A 和Q3B 质量浓度的提高和处理时间的延长,小枝的O₂ ^-· 产生速率、H2 O2 和MDA 含量均显著增加且总体上显著高于对照,仅各指标峰值出现的时间有差异,且用400 mg·L^-1 Q3A 和Q3B 处理后O₂ ^-· 产生速率、H2 O2 和MDA 含量均最高。随处理时间延长,各处理组SOD、CAT 和POD 活性均呈先增后降的变化趋势,仅峰值出现的时间有差异;各处理组处理12 ~60 h 时SOD 和CAT 活性和处理12 ~48 h 时POD 活性均显著高于对照;用400 mg·L^-1 Q3A 或Q3B 处理12 h 或24 h 时SOD、CAT 和POD 活性均最高;总体上看,随Q3A 和Q3B 质量浓度提高,SOD、CAT 和POD 活性在处理的前、中期逐渐增加,在处理的中、后期均呈先增后降的变化趋势。不同浓度Q3B 处理后木麻黄活性氧代谢水平及SOD 和CAT 活性高于相应浓度Q3A 处理,POD 活性则低于相应浓度Q3A 处理,表明Q3A 和Q3B 对木麻黄的化感机制存在差异。

The dynamic change of reactive oxygen metabolism and protective enzyme activity in branchlet of Casuarina equisetifolia Forst. seedling treated by 0(CK), 12. 5, 25, 50, 100, 200 and 400 mg·L^-1 quercetin-3-α-araboside ( Q3A) and quercetin-3-β-glucoside ( Q3B) for 0 - 72 h was studied by hydroponics. The results show that with rising of mass concentrations of Q3A and Q3B and prolonging of treatment time, O₂ ^-·  production rate and contents of H2O2 and MDA in branchlet all significantly increase and generally are significantly higher than those of the control, only appearance time of peak value of every index is various. And O₂ ^-·  production rate and contents of H2 O2 and MDA treated by 400 mg·L^-1  Q3A and Q3B are the highest. With prolonging of treatment time, activities of SOD, CAT and POD in all treatment groups appear the trend of increasing firstly and then decreasing, only appearance time of peak value of every index is various. Activities of SOD and CAT treated for 12-60 h and POD activity treated for 12-48 h in all treatment groups are higher than those of the control. Activities of SOD, CAT and POD treated by 400 mg·L^-1 Q3A or Q3B for 12 h or 24 h all are the highest. Generally, with rising of mass concentrations of Q3A and Q3B, activities of SOD, CAT and POD gradually increase at the early and middle stages, while appear the trend of increasing firstly and then decreasing at the middle and late stages. After treated by Q3B with different concentrations, reactive oxygen metabolism level and activities of SOD and CAT all are higher and POD activity is lower than those of treatment groups treated by Q3A with same concentrations, meaning that allelopathy mechanism of Q3A and Q3B to C. equisetifolia is different.