采用水培法,研究了Cu 单一处理及Cu 与EGTA 和丹皮酚磺酸钠(SPS)复合处理对凤丹(Paeonia suffruticosa ‘Feng Dan’)幼苗根长、根系中Cu 和丹皮酚含量的影响,并研究了Cu 和SPS 单一及复合处理对幼苗根系离体质膜和液泡膜微囊H⁺ -ATPase 活性的影响。结果显示:经5 μmol·L^-1 Cu 处理后凤丹幼苗根长和丹皮酚含量均略高于对照但总体上差异不显著; 经10、20 和30μmol·L^-1 Cu 处理后,幼苗根长和丹皮酚含量总体上均低于对照,且随Cu 浓度增加和处理时间延长,降幅增大。与10μmol·L^-1 Cu 单一处理相比,10μmol·L^-1  Cu-10μmol·L^-1  EGTA和10μmol·L^-1  Cu-10μmol·L^-1  SPS 复合处理均可以使根系中Cu 含量显著降低、丹皮酚含量显著提高;其中,10μmol·L^-1  Cu-10μmol·L^-1  EGTA 处理组幼苗根系中Cu 含量的降低幅度最大,而10μmol·L^-1  Cu-10μmol·L^-1 SPS 处理组幼苗根系中丹皮酚含量的增加幅度最大且显著高于对照。与对照相比,经5、10 和20μmol·L^-1 Cu 单一处理后丹凤根系离体质膜和液泡膜微囊H⁺ -ATPase 活性均降低, 且随Cu 浓度提高降低幅度增大; 而经0. 1、0. 2、0. 5 和1. 0μmol·L^-1  SPS 单一处理总体上可使膜微囊H⁺ -ATPase 活性逐渐增加;与10μmol·L^-1 Cu 单一处理相比,10μmol·L^-1  Cu 与0. 1、0. 2 和0. 5μmol·L^-1  SPS 复合处理均可使膜微囊H⁺ -ATPase 活性提高,且H⁺ -ATPase 活性均呈现随SPS 浓度提高逐渐增加的趋势。研究结果揭示:较高浓度Cu 胁迫对凤丹幼苗根系生长及丹皮酚合成以及质膜和液泡膜微囊H⁺ -ATPase 活性均有明显抑制作用,但添加外源丹皮酚磺酸钠对Cu 胁迫伤害具有一定的缓解效应。

Effects of Cu single treatment and complex treatments of Cu with EGTA and sodium paeonol sulfonate (SPS) on root length and contents of Cu and paeonol in root of Paeonia suffruticosa ‘Feng Dan’seedling were studied by water culture method, and effects of single and complex treatments of Cu and SPS on H⁺-ATPase activity of microcapsules in vitro of plasma membrane and tonoplast in root of P.suffruticosa ‘Feng Dan’seedling were also studied. The results show that root length and paeonol content in root of P. suffruticosa ‘Feng Dan’seedling treated by 5μmol·L^-1 Cu are slightly higher than those of the control but without significant difference generally. After treated by 10, 20 and 30μmol·L^-1  Cu, root length and paeonol content are generally lower than those of the control and the decrease range enhances gradually with rising of Cu concentration and prolonging of treatment time. Comparing to 10μmol·L^-1  Cu single treatment, complex treatments of 10μmol·L^-1 Cu-10μmol·L^-1  EGTA and 10μmol·L^-1 Cu-10μmol·L^-1 SPS can cause significantly decrease of Cu content in root, but significantly increase of paeonol content in root. In which, decrease range of Cu content in 10μmol·L^-1 Cu-10μmol·L^-1  EGTA treatment group is the highest and increase range of paeonol content in 10μmol·L^-1 Cu-10μmol·L^-1  SPS treatment group is the highest and significantly higher than that in the control. Comparing to the control, H+-ATPase activity of microcapsules in vitro of plasma membrane and tonoplast decreases after treated by single treatment with 5, 10 and 20μmol·L^-1 Cu and decrease range increases with rising of Cu concentration. But generally, single treatment with 0. 1, 0. 2, 0. 5 and 1. 0μmol·L^-1  SPS can gradually enhance H⁺-ATPase activity. Compared with 10μmol·L^-1  Cu single treatment, complex treatments of 10μmol·L^-1  Cu with 0. 1, 0. 2 and 0. 5μmol·L^-1  SPS all can generally enhance H+-ATPase activity with a gradually increasing trend with rising of SPS concentration. It is suggested that Cu stress with relative high concentration has an obvious inhibition to root growth, paeonol synthesis and H⁺-ATPase activity of microcapsules of plasma membrane and tonoplast in root of P. suffruticosa ‘Feng Dan’seedling, while adding exogenous sodium paeonol sulfonate has a mitigative effect on Cu stress injury.