2024年4月29日 星期一
微生物菌剂对芦竹修复Pb污染土壤的影响
Effects of microbial agent on remediation of Pbcontaminated soil by Arundo donax
2023年 第32卷 第6期 页码[43-49]    下载全文[1MB]  
摘要

了探明微生物菌剂对芦竹(Arundo donax Linn.)修复Pb污染土壤的影响,以ETS微生物菌剂为供试微生物菌剂,将对照组(未添加微生物菌剂和Pb)、微生物菌剂单一处理组(每个水泥池200 mL微生物菌剂)、Pb单一处理组(土壤Pb终质量浓度300 mg·kg-1)和微生物菌剂-Pb复合处理组(每个水泥池200 mL微生物菌剂,土壤Pb终质量浓度300 mg·kg-1)芦竹的生长情况和光合特性以及Pb的积累和迁移特征进行比较。结果显示:Pb单一处理组芦竹的株高、分蘖数和单丛地上部干质量显著(P<0.05)低于对照组,叶绿素相对含量、净光合速率和水分利用效率与对照组差异不显著(P>0.05),气孔导度、胞间CO2浓度和蒸腾速率显著高于对照组;而微生物菌剂-Pb复合处理组芦竹的株高、分蘖数、单丛地上部干质量和水分利用效率显著高于Pb单一处理组,叶绿素相对含量和胞间CO2浓度与Pb单一处理组差异不显著,净光合速率、气孔导度和蒸腾速率显著低于Pb单一处理组。Pb单一处理组芦竹根和茎的Pb含量显著高于对照组,叶的Pb含量和根的Pb富集系数与对照组差异不显著,茎和叶的Pb富集系数显著低于对照组;而微生物菌剂-Pb复合处理组芦竹根和茎的Pb含量以及根、茎和叶的Pb富集系数不显著高于Pb单一处理组,叶的Pb含量显著高于Pb单一处理组。从不同器官看,Pb单一处理组芦竹根的Pb含量和Pb富集系数最高,而微生物菌剂-Pb复合处理组芦竹叶的Pb含量和Pb富集系数最高。另外,芦竹的Pb转运系数在4个处理组间差异显著,其中,Pb单一处理组芦竹的Pb转运系数最低,微生物菌剂-Pb复合处理组芦竹的Pb转运系数显著高于Pb单一处理组。研究结果显示:微生物菌剂能够促进Pb胁迫下芦竹的生长,提高芦竹的Pb吸收和转运能力,进而增强其对Pb污染土壤的修复能力。

Abstract

To figure out the effects of microbial agent on remediation of Pb-contaminated soil by Arundo donax Linn., taking ETS microbial agent as the test microbial agent, the growth and photosynthetic characteristics as well as Pb accumulation and translocation characteristics of A. donax in the control group (not adding microbial agent and Pb), single treatment group of microbial agent (200 mL microbial agent per cement tank), single treatment group of Pb (300 mg·kg-1 of final mass concentration of Pb in soil), and combined treatment group of microbial agent-Pb (200 mL microbial agent per cement tank, 300 mg·kg-1 of final mass concentration of Pb in soil) were compared. The results show that plant height, tiller number, and dry mass of above-ground part per clump of A. donax in single treatment group of Pb are significantly (P<0.05) lower than those in the control group, relative chlorophyll content, net photosynthetic rate, and water use efficiency are not significantly (P>0.05) different from those in the control group, and stomatal conductance, intercellular CO2 concentration, and transpiration rate are significantly higher than those in the control group; while plant height, tiller number, dry mass of above-ground part per clump, and water use efficiency of A. donax in combined treatment group of microbial agent-Pb are significantly higher than those in single treatment group of Pb, relative chlorophyll content and intercellular CO2 concentration are not significantly different from those in single treatment group of Pb, and net photosynthetic rate, stomatal conductance, and transpiration rate are significantly lower than those in single treatment group of Pb. Pb contents in root and stem of A. donax in single treatment group of Pb are significantly higher than those in the control group, Pb content in leaf and Pb concentration coefficient in root are not significantly different from those in the control group, and Pb concentration coefficients in stem and leaf are significantly lower than those in the control group; while Pb contents in root and stem and Pb concentration coefficients in root, stem, and leaf of A. donax in combined treatment group of microbial agent-Pb are not significantly higher than those in single treatment group of Pb, and Pb content in leaf is significantly higher than that in single treatment group of Pb. In terms of different organs, Pb content and Pb concentration coefficient in root of A. donax in single treatment group of Pb are the highest, while those in leaf of A. donax in combined treatment group of microbial agent-Pb are the highest. In addition, Pb translocation coefficient of A. donax is significantly different between four treatment groups, in which, Pb translocation coefficient of A. donax in single treatment group of Pb is the lowest, and that in combined treatment group of microbial agent-Pb is significantly higher than that in single treatment group of Pb. It is suggested that microbial agent can promote the growth of A. donax under Pb stress, elevate its Pb absorption and translocation ability, and thus enhance its capacity to remediate Pbcontaminated soil.

关键词芦竹; 微生物菌剂; Pb污染土壤; 植物修复
Key wordsArundo donax Linn.; microbial agent; Pbcontaminated soil; phytoremediation
作者王柯懿, 陈霞霞, 莫凌, 曾丹娟, 蒲高忠
所在单位广西壮族自治区中国科学院广西植物研究所 广西喀斯特植物保育与恢复生态学重点实验室, 广西 桂林 541006
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基金项目国家自然科学基金项目(31660154); 中国科学院“西部之光”项目([2019]90); 广西重点研发计划项目(桂科AB22035038); 广西科学院基础研究基金项目(CQZ-D-1904); 广西喀斯特植物保育与恢复生态学重点实验室基金项目(20-065-7; 19-050-6)