运用开顶式气室(OTCs)模拟当前环境大气 O₃浓度(对照,40 ~ 45 nL· L^-1 )、O₃浓度倍增 1 倍( TR-1,92 ~106 nL· L^-1 )和 O₃浓度倍增 2 倍(TR-2,142 ~ 160 nL· L^-1 )胁迫,以叶片光合色素、MDA、可溶性蛋白质和可溶性糖含量及 SOD 和 POD 活性和相对电导率为指标,分析了美丽箬竹(Indocalamus decorus Q. H. Dai )对 O₃胁迫的生理响应规律。 结果显示:随 O₃ 浓度的提高,叶片叶绿素 a( Chla)、叶绿素 b( Chlb)、总叶绿素和类胡萝卜素含量,SOD 和 POD 活性,可溶性蛋白质和可溶性糖含量及二者的比值( SPC / SSC) 均呈下降趋势;叶绿素 a 与 b 的比值(Chla / Chlb)、MDA 含量和相对电导率均呈增加趋势。 TR-1 条件下叶片 POD 活性和可溶性糖含量分别比对照下降 38. 86% 和 10. 37% ,差异显著;Chla / Chlb 比值显著高于对照,其余指标均与对照无显著差异。 而在 TR-2 条件下叶片 Chla、Chlb、总叶绿素和类胡萝卜素含量显著低于对照,降幅分别为 26. 89% 、40. 91% 、30. 47% 和 20. 37% ;SOD 和 POD 活性、可溶 性 蛋 白 质 和 可 溶 性 糖 含 量 以 及 PC / SSC 比值也均显著低于对照,降幅分别为 42. 03% 、46. 62% 、45. 09% 、11. 52% 和 40. 15% ;Chla / Chlb 比值、MDA 含量和相对电导率均显著高于对照。另外,TR-1 与TR-2 处理组间光合色素含量和 POD 活性差异不显著,TR-2 处理组 MDA 含量和相对电导率显著高于 TR-1 处理组,SOD 活性显著低于后者。 研究结果表明:美丽箬竹对 O₃胁迫具有强耐受性,可种植于 O₃浓度较高的环境中。

 

The method of open-top chambers (OTCs) was used to simulate ozone stress with atmosphericO ₃ concentration (control, 40-45 nL· L^-1), one time O₃ concentration (TR-1, 92-106 nL· L^-1 ) and two times O ₃ concentration (TR-2, 142-160 nL· L^-1 ), and taking contents of photosynthetic pigment, MDA, soluble protein and soluble sugar, activities of SOD and POD and relative electric conductivity as indexes, physiological response of Indocalamus decorus Q. H. Dai to O₃ stress was researched. The results show that with rising of O₃ concentration, contents of chlorophylla (Chla), chlorophyllb (Chlb), total chlorophyll and carotenoid, activities of SOD and POD, soluble protein content (SPC) and soluble sugar content (SSC) and SPC / SSC value in leaves of I. decorus appear the reducing trend, while Chla / Chlb value, MDA content and relative electric conductivity appear the enhancing trend. In TR-1 group, POD activity and SSC decrease respectively 38. 86% and 10. 37% comparing to the control with the significant difference, Chla / Chlb value is significantly higher than that of the control, but the other indexes have no significant difference with the control. In TR-2 group, contents of Chla, Chlb, total chlorophyll and carotenoid are significantly lower than those of the control with decreasing ranges of 26. 89% , 40. 91% , 30. 47% and 20. 37% , respectively, activities of SOD and POD, SPC and SSC and SPC / SSC value also are significantly lower than those of the control with decreasing ranges of 42. 03% , 46. 62% , 45. 09% , 11. 52% and 40. 15% , respectively, but Chla / Chlb value, MDA content and relative electric conductivity are significantly higher than those of the control. Moreover,photosynthetic pigment content and POD activity have no significant differences between TR-1 and TR-2 groups, but MDA content and relative electric conductivity in TR-2 group are significantly higher and SOD activity in TR-2 group is significantly lower than those in TR-1 group. It is revealed that I. decorus possesses a strong resistance to O₃ stress, and can be cultivated in high O₃ concentration environment.