以结缕草品种‘Zenith’(Zoysia japonica‘Zenith’)的种子为外植体,在附加1.0 mg.L^-12,4-D和0.1mg.L^-16-BA或2.0 mg.L^-12,4-D的MS培养基上培养,愈伤组织诱导率较高,分别达到100%和92%。在添加了2.0 mg.L^-12,4-D或1.0 mg.L^-12,4-D和0.1 mg.L^-16-BA的MS培养基上继代培养可诱导出胚性愈伤组织,诱导率分别为3.26%和14.52%。胚性愈伤组织在含0.01mg.L^-12,4-D的1/2 MS分化培养基上的分化率和生根率都达到100%。 

Mature seeds of Zoysia japonica `Zenith' were used as initial explants for callus induction.The calli were induced atrelatively higher percentage of 92% and 100% respectively in MS medium containing 2.0mg·L^-1 2.4-Dor 1.0mg·L^-1 2,4-Dand 0.1mg·L^-1 6-BA. The embryo genic callus were induced at the MS medium containing 2.0mg·L^{- 1} 2,4-D or 1.0mg·L^{- 1} 2,4-D and 0.1mg·L^-1 6-BA with induced rates of 3.26% and 14.52% respectively. The rates of shoot regeneration and rooting both reached 100% at the medium of 1/2MS containing 0.01mg·L^{- 1} 2,4-D.