2021年12月7日 星期二
Pb 胁迫条件下狭叶香蒲种子的萌发特性及其幼苗的生理响应
Seed germination characteristics and seedling physiological response of Typha angustifolia under Pb stress
2014年 第23卷 第3期 页码[74-80]    下载全文[0.8MB]  
摘要

对 0、30、150、300、450 和 600 μmol· L-1 Pb 胁迫条件下狭叶香蒲(Typha angustifolia Linn.)种子的萌发特性进行了研究,并分析了 0、450、900、1 800 和 2 700 μmol· L-1 Pb 胁迫对狭叶香蒲幼苗叶片及根系中部分生理生化指标的影响。 结果表明:随 Pb 浓度提高,狭叶香蒲种子的发芽率、发芽势、发芽指数和活力指数以及下胚轴长度均逐渐下降且低于对照,而其下胚轴长度抑制指数则逐渐增大,但在 30 μmol· L-1 Pb 胁迫条件下各项萌发指标均与对照无显著差异。 叶片叶绿素 a、叶绿素 b 及总叶绿素含量随 Pb 浓度提高呈逐渐下降趋势,但在 450 和 900 μmol· L-1Pb 胁迫条件下与对照无显著差异,而在 1 800 和 2 700 μmol· L-1 Pb 胁迫条件下显著低于对照。 在 Pb 胁迫条件下叶片和根中 SOD 活性均显著高于对照但变化趋势不同;随 Pb 浓度提高,叶片 SOD 活性呈波动但整体上升的趋势,而根中 SOD 活性则呈逐渐降低的趋势。 叶片和根中 POD 活性均随 Pb 浓度提高呈持续上升的趋势,其中,在 450和 900 μmol· L-1 Pb 胁迫条件下叶片的 POD 活性低于对照、根的 POD 活性高于对照,但均与对照无显著差异;而在1 800 和 2 700 μmol· L-1 Pb 胁迫条件下叶片和根的 POD 活性均显著高于对照。 在 Pb 胁迫条件下叶片和根中 AsA和 MDA 含量均高于对照。 随 Pb 浓度提高,叶片的 AsA 含量总体上逐渐增加但在 450 和 900 μmol· L-1 Pb 胁迫条件下与对照无显著差异;而根的 AsA 含量则呈先增加后降低的趋势且均与对照差异显著。 随 Pb 浓度提高,叶片的MDA 含量先增后降但均与对照无显著差异;而根的 MDA 含量呈“高—低—高”的波动趋势且仅在 450 μmol· L-1Pb 胁迫条件下与对照差异显著。 综合分析结果显示:狭叶香蒲幼苗根系对 Pb 胁迫的敏感性可能强于叶片;狭叶香蒲种子可在轻度 Pb 污染水体中萌发和生长;其幼苗对 Pb 胁迫具有一定的耐性,可用于中度 Pb 污染水体的修复。

 

Abstract

Seed germination characteristics of Typha angustifolia Linn. under Pb stress with concentrations of 0, 30, 150, 300, 450 and 600 μmol· L-1 were studied, and effects of Pb stress with concentrations of 0, 450, 900, 1 800 and 2 700 μmol · L-1 on some physiological and biochemical indexes in leaf and root of seedling were analyzed. The results show that with rising of Pb concentration, germination rate, germination energy, germination index and vigor index and hypocotyl length of T. angustifolia seed all decrease gradually and are lower than those of the control, while inhibition index of hypocotyl length increases gradually, but under 30 μmol· L-1Pb stress, there is no significant difference in each germination index with the control. Contents of chla, chlb and total chlorophyll in leaf appear the trend of decreasing gradually with rising of Pb concentration, but those under 450 and 900 μmol· L-1 Pb stress have no significant differences comparing with those of the control, while those under 1 800 and 2 700 μmol· L-1Pb stress are significantly lower than those of the control. Under Pb stress, SOD activity in leaf and root is significantly higher than that of the control but their variation trend is different. With rising of Pb concentration, SOD activity in leaf appears a fluctuant but totally increasing trend, while that in root appears a gradually decreasing trend. POD activity in leaf and root appears a continually increasing trend with rising of Pb concentration. In which, under 450 and 900 μmol· L-1 Pb stress, POD activity in leaf is lower and that in root is higher than that of the control but with no significant difference to the control, while POD activity in leaf and root under 1 800 and 2 700 μmol· L-1Pb stress is significantly higher than that of the control. Under Pb stress, contents of AsA and MDA in leaf and root are higher than those of the control. With rising of Pb concentration, overall, AsA content in leaf increases gradually but that under 450 and 900 μmol · L-1 Pb stress has no significant difference comparing with the control, while AsA content in root appears the trend of firstly increasing and then decreasing with a significant difference to the control. With rising of Pb concentration, MDA content in leaf firstly increases and then decreases but with no significant difference to the control, while MDA content in root appears the fluctuant trend of “high-low-high” and only that under 450 μmol· L-1 Pb stress has a significant difference comparing with the control. The comprehensive analysis result indicates that the sensitiveness of root of T. angustifolia to Pb stress is probably stronger than that of leaf. And T. angustifolia seed can germinate and grow in light Pb polluted water and its seedling has a certain tolerance to Pb stress, so it can be used for repairing middle Pb polluted water.


 

关键词狭叶香蒲; Pb 胁迫; 种子萌发特性; 生理响应; 污染水体修复
Key wordsTypha angustifolia Linn.; Pb stress; seed germination characteristics; physiological response; polluted water restoration
作者徐金波, 徐迎春, 赵慧, 陈叶清
所在单位南京农业大学园艺学院, 江苏 南京 210095
点击量470
下载次数265
基金项目江苏省自然科学基金资助项目(BK2011640)