用RAPD标记技术分析了30个不同化学型樟树〔Cinnamomum camphora(L.)Presl〕样本的遗传关系。14个引物共扩增出170个位点,其中多态位点161个,多态性位点百分率达94.71%,特异位点有15个。聚类结果显示,当λ=13时,30个樟树样本被分为4类,即芳樟型(主成分为芳樟醇)、脑樟型(主成分为樟脑)、桉樟型(主成分为1,8-桉叶油素)和黄樟型(主成分为黄樟醇),表明不同化学型樟树样本间的遗传关系与其叶精油的主成分有一定的相关性。

Genetic relationships among thirty samples of different chemotypes of Cinnamomum camphora (L.) Presl were studied by RAPD technique. 170 loci were amplified by fourteen primers, among which 161 loci were polymorphic loci. The polymorphic loci percent age was 94.71%, fifteen specific RAPD markers were detected among these loci. The results of cluster analysis showed that when λ= 13, thirty samples were divided into fourgroups: linalool-type, camphor-type, cineol-type and safrole-type, their main component was linalool, camphor, 1, 8-cineole and safrole respectively. It is indicated that the genetic relationships among different chemotypes of C. camphora relate to the main components of essential oil in leaf.