2024年2月25日 星期日
龙凤竹的组织培养
Tissue culture of Pedilanthus tithymaloides var. nanus
2008年 第17卷 第3期 页码[73-77]    下载全文[0.8MB]  
摘要

以龙凤竹〔Pedilanthus tithymaloides(L.)Poit.var.nanus Dressler〕茎段为外植体,研究了龙凤竹愈伤组织诱导、植株再生以及试管苗继代保存培养的培养条件。结果表明,龙凤竹茎段灭菌的最佳方法是用1.0g·L-1HgCl2处理4~10min;愈伤组织诱导与分化的最佳培养基为添加1.5mg·mL-16-BA和0.10~0.15mg·mL-1NAA的MS培养基(含有30g·L-1蔗糖和6g.L-1琼脂粉,pH5.78~pH5.80);试管苗生根的最佳培养基为含有0.2mg·mL-1NAA的生根培养基(1/2MS,含有15g.L-1蔗糖和6g.L-1琼脂粉,pH5.78~pH5.80),试管苗生根率可以达到93.3%;经过炼苗并移栽后,龙凤竹试管苗的成活率可达95.0%以上;龙凤竹试管苗的最佳继代保存培养条件为:在含有0.1mg·mL-1NAA的生根培养基中,于温度15℃、光照强度20μmol·m-2·s-1的条件下继代保存。此外,龙凤竹愈伤组织可以直接分化产生大量丛生芽,达到龙凤竹试管苗增殖的目的。 

Abstract

The culture conditions of callus induction, plantlet regeneration and subculture were studied using stems of Pedilanthus tithymaloides(L.)Poit. var. nanusDressler as explants. The results show that the optimal sterilization method of stem segments of P. tithymaloides var. nanus is soaking in 1.0 g·L- 1HgCl2 solution for 4- 10min. The optimal medium of callus induction is MS medium containing1.5mg·L- 1 6-BA, 0.10- 0.15mg·L- 1 NAA, 30g·L- 1 sucrose and 6g·L- 1agar powder (pH 5.78-pH 5.80). The optimal rooting medium with rooting rate of 93.3% is the rooting medium (1/2MS, containing 15g·L- 1 sucrose and 6g·L- 1agar powder, pH 5.78-pH 5.80) containing 0.2mg·L- 1NAA, and the survival rate of P. tithymaloides var. nanus plantlets reach above 95.0% after hardened and transplanted. The optimal subculture medium of P. tithymaloides var. nanus plantlets is the rooting medium containing 0.1mg·L- 1NAA, and the optimal temperature and light intensity in subculture process is 15℃ and 20μmol·m - 2·s- 1, respectively. Moreover, the callus of P. tithymaloides var. nanus can directly differentiate and form. a large amount of rosette buds, so the multiplication goal of P. tithymaloides var. nanus plantlets can be achieved.

关键词龙凤竹; 组织培养; 愈伤组织;
Key wordsPedilanthus tithymaloides(L.)Poit. var. nanus Dressler; tissue culture; callus
作者顾福根,孙丙耀,陈瑞卿
所在单位苏州大学生命科学学院,江苏苏州215123
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下载次数686
基金项目苏州市农业科技发展计划攻关项目(SNZ-0305);