2024年2月25日 星期日
留兰香组织培养及快速繁殖条件的优化
Condition optimization of tissue culture and rapid propagation of Mentha spicata
2007年 第16卷 第4期 页码[38-42]    下载全文[0.7MB]  
摘要

以留兰香(Menthas picata L.)茎尖为实验材料,对外植体消毒、不定芽增殖和试管苗移栽生根的最佳条件进行研究。结果表明,最佳外植体消毒方法为:用体积分数75%乙醇浸泡30s,再用1.0g·L-1HgCl2浸泡10min,培养7d后外植体生长状况良好。正交实验结果表明,在附加0.2mg·L-16-BA和0.02mg·L-1NAA的MS培养基中,留兰香不定芽的增殖倍数最高,试管苗生长状况最好。在含25mg·L-16-BA和50mg·L-1NAA的混合溶液中浸泡1h,移栽试管苗的生根率可达100%,且根较长。 

Abstract

The optimal conditions for disinfecting of explants, propagating of adventitious buds and rooting of transferring plant lets of Menthas picata L. were studied by using stem tips as explants. The results showed that the optimal disinfection method was to put explants in 75% alcohol for 30s, then in1.0g·L- 1HgCl2 for 10min.As a result of disinfection, growth status of explants was better after cultured for 7d. Orthogonal test results showed that proliferation time of adventitious buds was the highest and growth status of seedlings was the best in MS medium containing 0.2mg·L- 1 6-BA and 0.02mg·L-1 NAA. Rooting rate of transferring plant lets were 100% and their roots were longer when putting in solution containing 25mg·L- 1 6-BA and 50mg·L- 1NAA for 1h.

关键词留兰香; 组织培养; 快速繁殖
Key wordsMentha spicata L.; tissue culture; rapid propagation
作者王小敏,梁呈元,李维林
所在单位江苏省•中国科学院植物研究所(南京中山植物园),江苏南京210014
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基金项目国家“十一五”科技支撑计划项目(2006BAI06A12-12); 江苏省“十五”高科技研究项目(BG2005317);