以留兰香(Menthas picata L.)茎尖为实验材料,对外植体消毒、不定芽增殖和试管苗移栽生根的最佳条件进行研究。结果表明,最佳外植体消毒方法为:用体积分数75%乙醇浸泡30s,再用1.0g·L^-1HgCl₂浸泡10min,培养7d后外植体生长状况良好。正交实验结果表明,在附加0.2mg·L^-16-BA和0.02mg·L^-1NAA的MS培养基中,留兰香不定芽的增殖倍数最高,试管苗生长状况最好。在含25mg·L^-16-BA和50mg·L^-1NAA的混合溶液中浸泡1h,移栽试管苗的生根率可达100%,且根较长。 

The optimal conditions for disinfecting of explants, propagating of adventitious buds and rooting of transferring plant lets of Menthas picata L. were studied by using stem tips as explants. The results showed that the optimal disinfection method was to put explants in 75% alcohol for 30s, then in1.0g·L^{- 1}HgCl₂ for 10min.As a result of disinfection, growth status of explants was better after cultured for 7d. Orthogonal test results showed that proliferation time of adventitious buds was the highest and growth status of seedlings was the best in MS medium containing 0.2mg·L^{- 1} 6-BA and 0.02mg·L^-1 NAA. Rooting rate of transferring plant lets were 100% and their roots were longer when putting in solution containing 25mg·L^{- 1} 6-BA and 50mg·L^{- 1}NAA for 1h.