Contents of neutral and polar lipids and fatty acid composition in seeds of Cornus officinalis Sieb. et Zucc. From Lin’an of Zhejiang, Xixia of He’nan and Foping, Danfeng, Taibai of Shaanxi were analyzed by GC-MS technique. The  determination results show that total contents of neutral and polar lipids in seeds from different locations are 31. 89% -36. 03% and 7. 96% -14. 89% , respectively. Both are mainly composed of linoleic acid, oleic acid, palmitic acid andstearic acid, in which, their relative contents in neutral lipids are 60.24% -64.57% , 22.32% -25.00% , 6.38% -6.88% and 2.56% -3. 58% , respectively, those in polar lipids are 59.06% -6.76% , 14.82% -17.06% , 6.31% -8.23% and 2.54%-3.88% , respectively. Overall, content of unsaturated fatty acids in oil of C. officinalis from Danfengof Shaanxi is higher. It is indicated that seeds of C. officinalis are rich in linoleic acid and oleic acid, which is a potential natural resource for extraction of excellent oil.