为深入探究斜生四链藻（Tetradesmus obliquus Wynne）源生物刺激剂对盐胁迫下小麦（Triticum aestivum Linn.）种子萌发及幼苗生长的影响，以蒸馏水为对照组，测定在盐胁迫（100 mmol·L^-1NaCl）下外源施加0、25、50、100和200 mg·L^-1斜生四链藻细胞提取液（MAE）后小麦种子萌发参数及幼苗生长和生理生化指标，利用主成分分析和隶属函数对不同质量浓度MAE的作用效果进行综合评价。结果表明：MAE可显著（P＜0.05）促进盐胁迫下小麦种子萌发，以质量浓度25 mg·L^-1促进效果最佳，分别使盐胁迫下的发芽率、发芽势和发芽指数提高了18.26%、34.52%和24.90%。MAE整体上可显著提升盐胁迫下小麦幼苗生长，以质量浓度50 mg·L^-1效果最佳，分别使盐胁迫下的茎长、根长、茎鲜质量、根鲜质量、茎干质量、根干质量提高了27.74%、11.70%、13.40%、37.42%、22.58%、53.66%。盐胁迫使小麦幼苗叶片的渗透调节物质含量较对照组显著上升，MAE处理使其进一步升高，且在50 mg·L^-1MAE处理下最高。与对照组相比，盐胁迫及MAE处理对叶片叶绿素b含量的影响不显著；盐胁迫使叶片叶绿素a含量显著降低，50 mg·L^-1MAE使叶片叶绿素a含量较盐胁迫组提高了31.65%；盐胁迫对叶片类胡萝卜素含量无显著影响，而MAE处理明显提高其含量，50 mg·L^-1MAE使叶片类胡萝卜素含量较盐胁迫组提高了60.27%。与对照组相比，盐胁迫使小麦幼苗根系膜透性和叶片丙二醛含量显著升高，MAE处理使二者有一定程度的降低，但整体上仍显著高于对照组；盐胁迫整体上显著提高了叶片抗氧化酶活性，MAE处理进一步提高了叶片抗氧化酶活性。综合评价结果显示50和25 mg·L^-1MAE对盐胁迫下小麦种子萌发和幼苗生长的促进效果较佳。综上，MAE可有效缓解盐胁迫对小麦种子萌发及幼苗生长的抑制作用，提高小麦的抗盐性。

To deeply investigate the effects of Tetradesmus obliquus Wynne-derived biostimulant on seed germination and seedling growth of Triticum aestivum Linn. under salt stress, 0, 25, 50, 100, and 200 mg·L^-1 T. obliquus cell extract （MAE） were exogenously applied under salt stress （100 mmol·L^-1 NaCl）, and distilled water was used as the control group, then the germination parameters of T. aestivum seeds as well as the growth and physiological and biochemical indexes of the seedlings were measured, and the effects of different mass concentrations of MAE were comprehensively evaluated by using principal component analysis and membership function. The results show that MAE can significantly （P<0.05） promote T. aestivum seed germination under salt stress, and the promoting effect is the best at the mass concentration of 25 mg·L^-1, which increases the germination rate, germination potential, and germination index under salt stress by 18.26%, 34.52%, and 24.90%, respectively. MAE can significantly enhance T. aestivum seedling growth under salt stress in general, and the effect is the best at the mass concentration of 50 mg·L^-1, which increases the stem length, root length, stem fresh mass, root fresh mass, stem dry mass, and root dry mass under salt stress by 27.74%, 11.70%, 13.40%, 37.42%, 22.58%, and 53.66%, respectively. Salt stress significantly increases the content of osmolytes in T. aestivum seedling leaves compared with the control group, and MAE treatment further elevates it, which is the highest under 50 mg·L^-1 MAE treatment. Compared with the control group, the effects of salt stress and MAE treatment on leaf chlorophyll b content are not significant; salt stress significantly decreases the leaf chlorophyll a content, while 50 mg·L^-1 MAE increases the leaf chlorophyll a content by 31.65% compared with the salt stress group; salt stress has no significant impact on leaf carotenoid content, but MAE treatment obviously increases its content, and 50 mg·L^-1 MAE increases the leaf carotenoid content by 60.27% compared with the salt stress group. Compared with the control group, salt stress significantly increases the root membrane permeability and leaf malonaldehyde content in T. aestivum seedlings, and MAE treatment results in a certain degree of reduction in both indexes, but they are still significantly higher than those in the control group in general. Salt stress significantly increases the leaf antioxidant enzyme activities in general, and MAE treatment further enhances the leaf antioxidant enzyme activities. The comprehensive evaluation results show that 50 and 25 mg·L^-1 MAE have relatively good promoting effects on seed germination and seedling growth of T. aestivum under salt stress. In conclusion, MAE can effectively alleviate the inhibitory effects of salt stress on T. aestivum seed germination and seedling growth, and enhance salt resistance of T. aestivum.