Total flavonoids content in stems, leaves and seeds of seven cultivars of Fagopyrum tataricum (L.) Gaertn. And a cultivar of F. esculentum Moench were determined by UV-visible spectrophotometry. The results show that total flavonoids content in leaves is higher. Total flavonoids content among different cultivars of F. tataricum has a great difference. Among seven cultivars of F. tataricum, total flavonoids content is the highest in leaves and seeds of ‘Jiande Tartary Buckwheat’ with 106. 16 and 24. 09 mg·g^-1 , respectively, and in stems of ‘Dingbian Tartary Buckwheat’with 34. 65 mg·g^-1 . The comprehensive analysis result indicates that ‘Jiande Tartary Buckwheat’is the best germplasm resources of F. tataricum and worth to further extension.