以凤丹( Paeonia suffruticosa ‘ Feng Dan’) 实生苗为砧木嫁接的牡丹品种‘ 肉芙蓉’( P. suffruticosa ‘Roufurong’)5 年生嫁接苗为实验材料,分析了土壤0(CK)、300、600 和900 mg·kg-1 Cu 胁迫条件下其部分生长和生理指标以及不同部位矿质元素含量,并比较了嫁接苗不同部位对Cu 的富集和转运能力。结果表明:经不同质量浓度Cu 处理后嫁接苗新枝长度和一级侧根长度与对照无显著差异(P>0. 05),一级侧根数量均显著少于对照(P<0. 05);而叶面积、比叶质量和主根长度仅在900 mg·kg^-1 Cu 胁迫下显著低于对照,根茎直径则在600 mg·kg^-1  Cu胁迫下显著高于对照。在Cu 胁迫条件下叶片叶绿素a、叶绿素b 和总叶绿素含量均与对照无显著差异,SOD 活性则均高于对照;经300 mg·kg^-1 Cu 处理后POD 活性高于对照、MDA 含量和叶绿素a / b 比值低于对照,而经600 和900 mg·kg^-1 Cu 处理后POD 活性低于对照、MDA 含量高于对照、叶绿素a / b 比值则显著低于对照。经不同质量浓度Cu 处理后,根中Cu 含量均高于茎和叶并随Cu 质量浓度的提高逐渐增加且均显著高于对照,而叶片中Cu 含量均低于对照,茎中Cu 含量均高于对照;叶、茎和根中P、Ca、Fe、Mn 和Zn 含量以及茎和根中的Mg 含量或高于对照或低于对照但总体上差异不显著;叶片中Mg 含量均显著高于对照;叶片和根中K 含量均高于对照,茎中K 含量或高于对照或低于对照,其中,在900 mg·kg^-1 Cu 胁迫条件下叶和根中的K 含量均显著高于对照。根对Cu 的富集系数均最大,叶对Cu 的富集系数均最小;从茎至叶的Cu 转运系数均最大,从根至叶的Cu 转运系数均最小;且根、茎和叶对Cu 的富集系数以及由茎到叶、由根到叶、由根到茎的Cu 转运系数均显著低于对照。综合分析结果显示:以凤丹为砧木的牡丹品种‘肉芙蓉’嫁接苗对Cu 胁迫具有一定的耐性,可用于轻度Cu 污染土壤的修复。

Using 5-year-old grafted seedlings of Paeonia suffruticosa ‘Roufurong’whose rootstock is P. suffruticosa ‘Feng Dan’seedling as experimental materials, some growth and physiological indexes, and mineral element content in different organs under stress conditions of 0 (CK), 300, 600 and 900mg·kg^-1 Cu in soil were analyzed and Cu enrichment and translocation abilities of different parts of grafted seedlings were compared. The results show that after treated by Cu with different mass concentrations, the lengths of new branch and primary lateral root of grafted seedlings have no significant difference (P>0. 05) with those of the control, primary lateral root number is significantly less than that of the control (P<0. 05). But only under 900 mg·kg-1 Cu stress, leaf area, specific leaf weight and main root length are significantly lower than those of the control, rhizome diameter under 600 mg·kg^-1 Cu stress is significantly higher than that of the control. Under Cu stress conditions, contents of Chla,Chlb and total chlorophyll in leaf all have no significant difference with those of the control, while SOD activity is higher than that of the control. After treated by 300 mg·kg^-1 Cu, POD activity is higher and MDA content and ratio of Chla / Chlb are lower than those of the control, while after treated by 600 and 900 mg·kg^-1  Cu, POD activity is lower and MDA content is higher than those of the control, and ratio of  Chla / Chlb is significantly lower than that of the control. After treated by Cu with different mass concentrations, Cu content in root is higher than that in stem and leaf, and increases gradually with rising of Cu mass concentration and is significantly higher than that of the control, but Cu content in leaf is lower and that in stem is higher than those of the control. Contents of P, Ca, Fe, Mn and Zn in leaf, stem and root, and Mg content in stem and root are higher or lower than those of the control but with no significant difference generally. Mg content in leaf is significantly higher than that of the control. K content in leaf and root is higher than that of the control, and that in stem is higher or lower than that of the control, in which, K content in leaf and root under 900 mg·kg^-1 Cu stress condition is significantly higher than that of the control. Cu enrichment coefficient of root is the maximum, and that of leaf is the minimum. Cu translocation coefficient from stem to leaf is the maximum, and that from root to leaf is the minimum. And Cu enrichment coefficient of root, stem and leaf and Cu translocation coefficient from stem to leaf, root to leaf and root to stem all are significantly lower than those of the control. The result of comprehensive analysis shows that the seedling of P. suffruticosa ‘Roufurong’grafted by P. suffruticosa ‘Feng Dan’ as rootstock has a certain resistance to Cu, which can be used for repairing the soil polluted slightly by Cu.