2024年7月21日 星期日
用盾叶薯蓣生产薯蓣皂苷元预发酵与水解条件优化
Optimization of spontaneous fermentation and acid hydrolysis of extracting diosgenin from the rhizome of Dioscorea zingiberensis
2004年 第13卷 第3期 页码[35-37]    下载全文[0.5MB]  
摘要

采用RP HPLC法检测薯蓣皂苷元,对薯蓣皂苷元提取过程中影响产率的多种因素,分预发酵、水解2部分分别用单因素和正交设计的方法进行优化。结果表明:40℃预发酵16h, 加6%浓H2SO4, 料液比为1∶6, 温度为121~126℃,水解5h, 产率高达3.62%,说明预发酵与水解条件优化可以提高盾叶薯蓣(Dioscorea zingiberensis C. H. Wright)生产薯蓣皂苷元的产率。

Abstract

Tooptimize the termof extracting technology of diosgenin fromthe rhizome of Dioscorea zingiberensis C.H.Wright, single factor and orthogonal tests were applied by optimization of extracting technology, the diosgenin was determined by RP-PHLC. The results showed that the optimization terms of spontaneous fermentation is at40℃ ,16 h, acid hydrolysis term6% H2SO4,proportion of solid to liquid 1∶6, temperature121- 126℃ , hydrolysis time 5 h. Using these conditions extracting diosgenin from the rhizome of D. zingiberensis, the diosgenin content increased to 3.62%. It suggests the optimization of spontaneous fermentation and acid hydrolysis can improve the production of diosgenin from the rhizome of D. zingiberensis.

关键词盾叶薯蓣; 薯蓣皂苷元; 预发酵; 酸水解; 优化
Key wordsDioscorea zingiberensis C. H. Wright; diosgenin; spontaneous fermentation; acid hydrolysis; optimization
作者汤兴利,徐增莱,夏冰,史云云
所在单位江苏省药用植物研究开发中心,江苏省中国科学院植物研究所(南京中山植物园),江苏南京210014
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