2021年12月6日 星期一
低温胁迫下不同种源香椿含水量和渗透调节物质含量差异及其与抗寒性的相关性
Differences in water and osmoregulation substance contents in Toona sinensis from different provenances under low temperature stress and their correlation to cold tolerance
2014年 第23卷 第4期 页码[47-54]    下载全文[0.8MB]  
摘要

以 来源于不同产地的10 个香椿[Toona sinensis (A. Juss.) Roem.]种 源 为 研 究 对 象、以温度 25 ℃为对照,对 5  ℃ 、0  ℃ 、-5  ℃和-10  ℃低温胁迫条件下叶片的水分含量指标(包括总含水量、自由水含量、束缚水含量和自由水含量与束缚水含量比值)和渗透调节物质含量(包括可溶性糖含量和脯氨酸含量)的变化进行了比较,并对这些指标与低温半致死温度(LT50 )的相关性进行了分析。 结果表明:在 5  ℃至-5  ℃ 条件下,各种源幼苗叶片的总含水量、自由水含量以及自由水含量与束缚水含量比值均低于对照,并且大多种源的上述指标随温度降低不断下降;各种源幼苗叶片的束缚水含量则均高于对照,并在 0  ℃条件下达到最高值;多数种源幼苗叶片的可溶性糖含量及脯氨酸含量则随温度降低而持续上升。 在-10  ℃条件下,所有种源幼苗叶片的总含水量和自由水含量以及绝大多数种源的自由水含量与束缚水含量比值均高于-5  ℃ 条件下,但束缚水含量、可溶性糖含量和脯氨酸含量则均低于-5  ℃条件下。 相关性分析结果表明:在5  ℃条件下,束缚水含量与 LT50呈显著负相关、自由水含量与束缚水含量比值与 LT50呈显著正相关,而在 0  ℃ 、-5  ℃和-10  ℃条件下这 2 个指标与 LT50则分别呈不显著的负相关和正相关;在5  ℃和-5  ℃条件下叶片的自由水含量与 LT50呈显著正相关,而在 0  ℃ 和-10  ℃ 条件下则呈不显著正相关;在 5  ℃条件下叶片的可溶性糖含量和脯氨酸含量与 LT50呈不显著负相关,而在 0  ℃ 、-5  ℃ 和-10  ℃ 条件下则与 LT50呈显著负相关。 研究结果表明:供试的 10 个香椿种源均不能耐受-10  ℃的低温胁迫,应在-5  ℃至-10  ℃范围内进一步研究确定其低温耐受阈值。

 

Abstract

Taking ten provenances of Toona sinensis ( A. Juss.) Roem. from different locations as researching subjects and temperature 25  ℃ as the control, changes in water content indexes ( including contents of total water, free water and bound water, and ratio of free water content to bound water content) and osmoregulation substance content ( including soluble sugar and proline contents) in leaf under low temperature stress conditions of 5  ℃ , 0  ℃ , - 5  ℃ and - 10  ℃ were compared, and correlations between these indexes and the low semilethal temperature (LT50) were analyzed. The results show that under conditions from 5  ℃ to -5  ℃ , total water content, free water content and ratio of free water content to bound water content in leaf of T. sinensis seedlings from different provenances all are lower than those of the control, and above indexes of most provenances decrease gradually with decreasing of temperature; bound water content in leaf of seedlings from different provenances is higher than that of the control and reaches the highest value under condition of 0  ℃ ; contents of soluble sugar and proline in leaf of seedlings from most provenances increase continuously with decreasing of temperature. Under condition of -10  ℃ , total water content and free water content in leaf of all provenances and ratio of free water content to bound water content in leaf of most provenances all are higher than those under condition of -5  ℃ , but contents of bound water, soluble sugar and proline all are lower than those under condition of -5  ℃ . The results of correlation analysis show that under condition of 5  ℃ , there is significantly negative correlation between bound water content and LT50, and there is significantly positive correlation between ratio of free water content to bound water content and LT 50, while under conditions of 0  ℃ , -5  ℃ and -10  ℃ , there is no significantly negative and significantly positive correlations between the two indexes and LT 50, respectively. There is significantly positive correlation between free water content in leaf and LT 50 under conditions of 5  ℃ and -5  ℃ , while there is no significantly positive correlation between them under conditions of 0  ℃ and -10  ℃ . There is no significantly negative correlation between contents of soluble sugar and proline and LT50 under condition of 5  ℃ , while there is significantly negative correlation between them under conditions of 0  ℃ , -5  ℃ and -10  ℃ . It is suggested that ten provenances tested of T. sinensis all are not tolerant to - 10  ℃ low temperature stress, the tolerance threshold of T. sinensis to lower temperature should be confirmed from -5  ℃ to -10  ℃ .

关键词香椿; 种源; 含水量; 渗透调节物质; 抗寒性; 相关性分析
Key wordsToona sinensis ( A. Juss.) Roem.; provenance; water content; osmoregulation substance; cold tolerance; correlation analysis
作者杨玉珍1, 陈刚1, 彭方仁2, 王国霞1, 罗青1, 马晓3
所在单位1. 郑州师范学院生命科学学院, 河南 郑州 450044;
2. 南京林业大学林学院, 江苏 南京 210037;
3. 河南职业技术学院环境艺术工程系, 河南 郑州 450046
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基金项目河南省科技攻关项目(112102110153); 郑州师范学院资助项目(2012080)