应用 ISSR 分子标记技术对来自黄河下游区域(山东省和河北省)的 46 个鲁桑[Morus alba var. multicaulis (Perrott.) Loud.]地方品种的遗传多样性进行了分析;并基于遗传相似系数、采用 UPGMA 法对这些地方品种的遗传关系进行了研究。 结果表明,用 15 个 ISSR 引物从 46 个鲁桑地方品种的总 DNA 中共扩增出 109 条带,其中多态性条带 81 条,多态性条带百分率为 74. 31% ,表明供试的鲁桑地方品种间存在丰富的遗传多样性;46 个地方品种间的遗传相似系数为 0. 602 ~ 0. 898,相对偏高。 聚类分析结果显示,46 个鲁桑地方品种被分为 2 类,其中,第 1 类包含来自河北的品种‘冀丰’和‘冀黄鲁选’,第 2 类包含其余 44 个品种;后者还能进一步分成 9 个亚类,其中 A、E、F、G、H 和 I 亚类所包含的 36 个品种均来自山东,组成 B、C 和 D 亚类的品种分别来自河北和山东;此外,E 亚类仅包含‘白条擗桑’1 个品种,是 46 个品种中惟一的三倍体品种。 研究结果显示,基于 ISSR 标记分析的 46 个鲁桑地方品种的遗传关系与地理分布具有一定的相关性。

 

Genetic diversity of forty-six local varieties of Morus alba var. multicaulis ( Perrott.) Loud. from the lower area of Yellow River ( Shandong Province and Hebei Province) was analyzed by ISSR molecular marker technique. And according to the genetic similarity coefficient, the genetic relationship among these local varieties was researched by UPGMA method. The results show that 109 bands are amplified from total DNA of forty-six local varieties using fifteen ISSR primers, in which, there are 81 polymorphic bands with a percentage of polymorphic band of 74. 31% . It means that there is rich genetic diversity among different tested local varieties. The genetic similarity coefficients among forty-six local varieties are relatively high with a range of 0. 602-0. 898. The cluster analysis results show that forty-six local varieties are divided into two categories, in which, ‘ Jifeng’ and ‘ Jihuangluxuan’ from Hebei Province are clustered to the first category, the other forty-four varieties are clustered to the second one.And the latter can be further divided into nine subcategories, in which, the thirty-six varieties belonging to A, E, F, G, H and I subcategories all originate from Shandong Province, the varieties belonging to B,C and D subcategories originate from Hebei Province and Shandong Province, respectively. Besides, subcategory E contains one variety ‘ Baitiaopisang’ which is only one triploid variety in forty-six local varieties. It is suggested that the genetic relationship of forty-six local varieties of M. alba var. multicaulis based on ISSR marker has a certain correlation with geographical distribution.