2021年10月17日 星期日
白及Aux/IAA基因的鉴定及分析
Identification and analysis on Aux/IAA genes of Bletilla striata
2020年 第29卷 第3期 页码[11-18]    下载全文[8.4MB]  
摘要

基于白及〔Bletilla striata (Thunb. ex A. Murray) Rchb. f.〕的全转录组数据,采用生物信息学方法对白及Aux/IAA基因进行鉴定和序列分析,对白及Aux/IAA蛋白的一级和二级结构、基序、保守结构域、GO功能分类和KEGG富集结果进行分析,并对其与拟南芥〔Arabidopsis thaliana (Linn.) Heynh.〕和铁皮石斛(Dendrobium officinale Kimura et Migo)的Aux/IAA蛋白进行系统发育分析。结果表明:在白及的全转录组中共筛选出5个Aux/IAA基因,分别命名为BsAux/IAA1至BsAux/IAA5,开放阅读框(ORF)长度为492~2 739 bp。5个BsAux/IAA蛋白的氨基酸残基数为163~912,理论相对分子质量为18 172.97~101 442.89,理论等电点为pI 4.40至pI 11.51,亚细胞定位于细胞核中,二级结构以α-螺旋和无规则卷曲为主。5个BsAux/IAA蛋白共有Domain Ⅰ、Domain Ⅱ、Domain Ⅲ和Domain Ⅳ 4个完整的保守结构域,依次对应保守基序motif9、motif3、motif2和motif1。5个BsAux/IAA蛋白的功能均包括细胞组分、生物学过程和分子功能3个大类,并参与植物激素信号转导通路。系统发育分析结果表明:5个BsAux/IAA蛋白均属于GroupⅠ亚家族,并进一步分成a和b 2个亚群,其中,BsAux/IAA3、BsAux/IAA4和BsAux/IAA5属于a亚群,BsAux/IAA1和BsAux/IAA2属于b亚群;并且,BsAux/IAA1、BsAux/IAA2、BsAux/IAA4和BsAux/IAA5与铁皮石斛Aux/IAA的亲缘关系较近。研究结果显示:白及Aux/IAA蛋白具有一定的保守性,可能在细胞核中发挥作用,并具有组织特异性、发育依赖性和非生物胁迫应答性。

Abstract

 Based on the whole transcriptome data of Bletilla striata (Thunb. ex A. Murray) Rchb. f., identification and sequence analysis on Aux/IAA genes of B. striata were conducted by using bioinformatics methods, and primary and secondary structures, motif, conserved domain, GO function classification, and KEGG enrichment result of Aux/IAA proteins of B. striata were analyzed, meanwhile phylogenetic analysis on them with Aux/IAA proteins of Arabidopsis thaliana (Linn.) Heynh. and Dendrobium officinale Kimura et Migo were performed. The results show that 5 Aux/IAA genes are screened from the whole transcriptome of B. striata, named as BsAux/IAA1 to BsAux/IAA5, respectively, with an open reading frame. (ORF) length of 492-2 739 bp. The amino acid residue numbers of 5 BsAux/IAA proteins are 163-912, with theoretical relative molecular mass of 18 172.97-101 442.89 and theoretical isoelectric point of pI 4.40 to pI 11.51, their subcellular locations are in the nucleus, and their secondary structures are mainly αhelix and random coil. There are 4 complete conserved domains of Domain Ⅰ, Domain Ⅱ, Domain Ⅲ, and Domain Ⅳ in 5 BsAux/IAA proteins, corresponding to conserved motifs of motif9, motif3, motif2, and motif1 in order. The functions of 5 BsAux/IAA proteins all include 3 categories of cellular component, biological process, and molecular function, and are involved in signal transduction pathways of plant hormones. The phylogenetic analysis result shows that 5 BsAux/IAA proteins all belong to Group Ⅰ subfamily, and can be divided into 2 subgroups of a and b, in which, BsAux/IAA3, BsAux/IAA4, and BsAux/IAA5 belong to a subgroup, while BsAux/IAA1 and BsAux/IAA2 do to b subgroup; in addition, there are close genetic relationships in BsAux/IAA1, BsAux/IAA2, BsAux/IAA4, and BsAux/IAA5 with Aux/IAA of D. officinale. It is suggested that there is a certain conservation in Aux/IAA proteins of B. striata, the proteins may play roles in nucleus, and possess tissue specificity, development dependence, and abiotic stress response.

关键词白及; Aux/IAA基因; 基因鉴定; 序列分析; 系统发育分析
Key wordsBletilla striata (Thunb. ex A. Murray) Rchb. f.; Aux/IAA gene; gene identification; sequence analysis; phylogenetic analysis
作者刘厚伯1, 李春2, 李林1, 上官艳妮1, 黄恻隐1, 刘怡1, 郑明辉1, 徐德林1
所在单位1. 遵义医科大学 细胞生物学教研室, 贵州 遵义 563099; 2. 河南省农业科学院 芝麻研究中心, 河南 郑州 450002
点击量857
下载次数127
基金项目国家自然科学基金资助项目(31560079; 31560102); 贵州省科学技术基金项目(黔科合平台人才[2017]5733-050; 黔科合平台人才[2017]5733-001); 贵州省中医药管理局项目(QZYY-2019-060); 贵州省教育厅人才项目(KY[2017]194)