基于白及〔Bletilla striata （Thunb. ex A. Murray） Rchb. f.〕的全转录组数据，采用生物信息学方法对白及Aux/IAA基因进行鉴定和序列分析，对白及Aux/IAA蛋白的一级和二级结构、基序、保守结构域、GO功能分类和KEGG富集结果进行分析，并对其与拟南芥〔Arabidopsis thaliana （Linn.） Heynh.〕和铁皮石斛（Dendrobium officinale Kimura et Migo）的Aux/IAA蛋白进行系统发育分析。结果表明：在白及的全转录组中共筛选出5个Aux/IAA基因，分别命名为BsAux/IAA1至BsAux/IAA5，开放阅读框（ORF）长度为492~2 739 bp。5个BsAux/IAA蛋白的氨基酸残基数为163~912，理论相对分子质量为18 172.97~101 442.89，理论等电点为pI 4.40至pI 11.51，亚细胞定位于细胞核中，二级结构以α-螺旋和无规则卷曲为主。5个BsAux/IAA蛋白共有Domain Ⅰ、Domain Ⅱ、Domain Ⅲ和Domain Ⅳ 4个完整的保守结构域，依次对应保守基序motif9、motif3、motif2和motif1。5个BsAux/IAA蛋白的功能均包括细胞组分、生物学过程和分子功能3个大类，并参与植物激素信号转导通路。系统发育分析结果表明：5个BsAux/IAA蛋白均属于GroupⅠ亚家族，并进一步分成a和b 2个亚群，其中，BsAux/IAA3、BsAux/IAA4和BsAux/IAA5属于a亚群，BsAux/IAA1和BsAux/IAA2属于b亚群；并且，BsAux/IAA1、BsAux/IAA2、BsAux/IAA4和BsAux/IAA5与铁皮石斛Aux/IAA的亲缘关系较近。研究结果显示：白及Aux/IAA蛋白具有一定的保守性，可能在细胞核中发挥作用，并具有组织特异性、发育依赖性和非生物胁迫应答性。

 Based on the whole transcriptome data of Bletilla striata （Thunb. ex A. Murray） Rchb. f.， identification and sequence analysis on Aux/IAA genes of B. striata were conducted by using bioinformatics methods， and primary and secondary structures， motif， conserved domain， GO function classification， and KEGG enrichment result of Aux/IAA proteins of B. striata were analyzed， meanwhile phylogenetic analysis on them with Aux/IAA proteins of Arabidopsis thaliana （Linn.） Heynh. and Dendrobium officinale Kimura et Migo were performed. The results show that 5 Aux/IAA genes are screened from the whole transcriptome of B. striata， named as BsAux/IAA1 to BsAux/IAA5， respectively， with an open reading frame. （ORF） length of 492-2 739 bp. The amino acid residue numbers of 5 BsAux/IAA proteins are 163-912， with theoretical relative molecular mass of 18 172.97-101 442.89 and theoretical isoelectric point of pI 4.40 to pI 11.51， their subcellular locations are in the nucleus， and their secondary structures are mainly αhelix and random coil. There are 4 complete conserved domains of Domain Ⅰ， Domain Ⅱ， Domain Ⅲ， and Domain Ⅳ in 5 BsAux/IAA proteins， corresponding to conserved motifs of motif9， motif3， motif2， and motif1 in order. The functions of 5 BsAux/IAA proteins all include 3 categories of cellular component， biological process， and molecular function， and are involved in signal transduction pathways of plant hormones. The phylogenetic analysis result shows that 5 BsAux/IAA proteins all belong to Group Ⅰ subfamily， and can be divided into 2 subgroups of a and b， in which， BsAux/IAA3， BsAux/IAA4， and BsAux/IAA5 belong to a subgroup， while BsAux/IAA1 and BsAux/IAA2 do to b subgroup； in addition， there are close genetic relationships in BsAux/IAA1， BsAux/IAA2， BsAux/IAA4， and BsAux/IAA5 with Aux/IAA of D. officinale. It is suggested that there is a certain conservation in Aux/IAA proteins of B. striata， the proteins may play roles in nucleus， and possess tissue specificity， development dependence， and abiotic stress response.