2024年2月25日 星期日
HPLC 法测定银杏叶片剂中总黄酮甙的含量
Determination of total flavonoid glycoside in Ginkgo biloba leaf tablet by HPLC
2001年 第10卷 第4期 页码[57-58]    下载全文[0.3MB]  
Abstract

Total flavonoid gly coside was isolated from Ginkgo biloba Linn.leaf tablet and determined by RP-HPLC using quercetin as external standard .4.6 mm ×100 mm Spherisorb ODS column, mobile phase:V( CH3OH) V( 0.3 %H3PO4) =4060, flow rate:1 mL/min, detection :λ=370 nm.The sample was extracted with CH3OH by ultrasonic extraction and hydrolyzed with 25 % HCl, then sample solution was diluted accurately to 50 mL.This experiment showed that this is a simple, accurate and precise method with the result of fine linearity, average recovery is 101.1 % for quercetin, and RSD is 2 .44%
 

关键词银杏; 银杏叶片剂; 槲皮素; 总黄酮甙; HPLC
Key wordsGinkgo biloba Linn.; Ginkgo biloba leaf tablet; quercetin; total flavonoid glycoside; HPLC
作者杨如同, 韩福贵, 潘福生
所在单位江苏省中国科学 院植物研究所, 江苏 南京 210014
点击量923
下载次数688