2024年7月23日 星期二
辣椒NAC家族成员鉴定及其编码基因在NaCl胁迫下的表达分析
Identification of NAC family members of Capsicum annuum and analysis on expressions of their coding genes under NaCl stress
2023年 第32卷 第4期 页码[12-24]    下载全文[17.5MB]  
摘要

 基于辣椒(Capsicum annuum Linn.)品种‘Zunla-1’全基因组数据,对辣椒NAC家族成员进行鉴定和生物信息学分析,并对250 mmol·L-1NaCl胁迫0、3和72 h辣椒盐敏感自交系XWHJ-M和耐盐自交系H1023叶中NAC基因的表达情况进行分析。结果表明:本研究鉴定出102个NAC家族成员,命名为CaNAC001至CaNAC102。这些家族成员的氨基酸残基数、理论相对分子质量、理论等电点和不稳定指数存在较大差异,均为亲水蛋白,且98%的家族成员没有跨膜结构域。亚细胞定位结果显示:91个家族成员位于细胞核,其他家族成员位于细胞质。辣椒和拟南芥〔Arabidopsis thaliana (Linn.) Heynh.〕的NAC家族成员可分成15组。辣椒NAC家族成员最多含有15个motif,多数成员含有motif3和motif5,且仅含有NAM结构域。辣椒NAC基因最多含有6个内含子,且这些基因分布在13条染色体上。有54对辣椒和拟南芥的NAC基因存在共线性。辣椒NAC家族成员互作网络有ATM、MYB46、NTL等10个核心蛋白,且多数成员间无互作关系。辣椒NAC基因启动子共有19种顺式作用元件,包括调节激素信号通路、非生物胁迫相关和生长发育相关顺式作用元件。基因表达谱热图显示:2个自交系叶中NAC基因的相对表达量在不同胁迫时间存在较大差异,并且CaNAC014、CaNAC020、CaNAC026、CaNAC075和CaNAC078基因的相对表达量在2个自交系间差异较大。综上所述,辣椒NAC家族成员较为保守,且与拟南芥NAC家族成员的同源性较高;辣椒NAC基因能够参与调节激素响应和非生物胁迫响应等生理过程。

Abstract

 Based on the whole genome data of Capsicum annuum Linn. cultivar ‘Zunla-1’, identification and bioinformatics analysis were conducted for NAC family members of C. annuum, and the expression patterns of NAC genes in leaves of saltsensitive inbred line XWHJM and salttolerant inbred line H1023 of C. annuum under 250 mmol·L-1 NaCl stress for 0, 3, and 72 h were analyzed. The results show that 102 NAC family members are identified in this research, namely CaNAC001 to CaNAC102. There are great differences in amino acid residue number, theoretical relative molecular mass, theoretical isoelectric point, and instability index of these family members, all of them are hydrophilic proteins, and there is no transmembrane domain in 98% of these family members. The subcellular localization result shows that 91 family members are localized in cell nucleus, while the other family members are localized in cytoplasm. The NAC family members of C. annuum and Arabidopsis thaliana (Linn.) Heynh. can be divided into 15 groups.  The NAC family members of C. annuum contain up to 15 motifs, most members contain motif3 and motif5, and only contain NAM domain. NAC genes of C. annuum contain up to 6 introns, and these genes are distributed in 13 chromosomes. There are collinearities of 54 pairs of NAC genes of C. annuum and A. thaliana. There are 10 core proteins including ATM, MYB46, NTL, etc. in  interaction network of NAC family members of C. annuum, and there is  no interaction between most members. There are 19 types of cis-acting elements in promoters of NAC genes of C. annuum, including hormone signal pathway regulatory, abiotic stress related, and growth and development related cis-acting elements. The heat map of gene expression profile shows that there are great differences in relative expression levels of NAC genes in leaves of the two inbred lines among different stress times; in addition, there are great differences in relative expression levels of CaNAC014, CANAC020, CaNAC026, CaNAC075, and CANAC078 between the two bred lines. Overall, NAC family members of C. annuum  are relatively conserved,  and there are high homologies between them and NAC family members of A. thaliana; the NAC genes of C. annuum can participate in regulation of biological processes, such as hormone response, abiotic stress response, etc.

关键词辣椒; NAC家族; 生物信息学分析; NaCl胁迫; 基因表达
Key wordsCapsicum annuum Linn.; NAC family; bioinformatics analysis; NaCl stress; gene expression
作者黄畋柳1, 张锐2, 贺迎骁1, 杨蓉芝1, 宋威威1, 赖钟雄1, 李宁3, 刘生财1
所在单位1. 福建农林大学园艺植物生物工程研究所, 福建 福州 350002; 2. 福建省(山区)作物遗传改良与创新利用重点实验室, 福建 三明 365500;3. 湖北省农业科学院经济作物研究所 蔬菜种质创新与遗传改良湖北省重点实验室, 湖北 武汉 430064
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基金项目福建省科技计划项目(2022N0036); 福建省(山区)作物遗传改良与创新利用重点实验室开放课题资助项目(2022SKF03); 福建农林大学经济作物种业工程服务队项目(11899170125)