采用盆栽法研究了根瘤菌Rhizobium trifolii ACCC18017对Cu添加量为0、500、1 000和1 500 mg·kg^-1土壤中白三叶（Trifolium repens Linn.）的株高、主根长、地上部和地下部的鲜质量和干质量、叶片叶绿素含量以及地上部和地下部的Cu含量的影响。结果表明：Cu添加量为500 mg·kg^-1土壤中对照组（接种灭菌的2.7×109 CFU·mL^-1菌悬液）白三叶的株高、地上部和地下部的鲜质量及地上部干质量均显著（P＜0.05）高于Cu添加量为0 mg·kg^-1的土壤；Cu添加量为1 000 mg·kg^-1土壤中对照组白三叶的株高、地上部鲜质量及地上部和地下部的干质量与Cu添加量为0 mg·kg^-1的土壤无显著差异；并且，Cu添加量为500、1 000和1 500 mg·kg^-1土壤中对照组白三叶的主根长和叶片叶绿素含量与Cu添加量为0 mg·kg^-1的土壤无显著差异。上述结果表明白三叶对一定程度的Cu污染土壤具有适应性。不同Cu添加量土壤中接菌组（先后接种2.7×109和2.3×109 CFU·mL^-1菌悬液）白三叶供试所有指标基本上均高于对照组；其中，Cu添加量为0、500和1 000 mg·kg^-1土壤中接菌组白三叶的株高及地上部和地下部的鲜质量和干质量基本上显著高于对照组。并且，Cu添加量为500和1 000 mg·kg^-1土壤中接菌组白三叶地上部和地下部的Cu含量显著高于对照组。本研究结果显示：根瘤菌R. trifolii ACCC18017能够促进白三叶生长及其对土壤中Cu的吸收，利于白三叶修复一定程度的Cu污染土壤。

Effects of Rhizobium trifolii ACCC18017 on height， main root length， fresh and dry masses of above and underground parts， chlorophyll content in leaf， and Cu content in above- and under-ground parts of Trifolium repens Linn. in soil with Cu additions of 0， 500， 1 000， and 1 500 mg·kg^-1 were studied by using potculture method. The results show that height， fresh masses of above- and under-ground parts， and dry mass of above-ground part of T. repens in the control group （inoculated with sterilized 2.7×109 CFU·mL^-1  suspension） in soil with Cu addition of 500 mg·kg^-1  are significantly （P＜0.05） higher than those of T. repens in soil with Cu addition of 0 mg·kg^-1； height， fresh mass of above-ground part， and dry masses of above- and under-ground parts of T. repens in the control group in soil with Cu addition of 1 000 mg·kg^-1 have no significant difference with those of T. repens in soil with Cu addition of 0 mg·kg^-1； in addition， main root length and chlorophyll content in leaf of T. repens in the control group in soil with Cu additions of 500， 1 000， and 1 500 mg·kg^-1 have no significant difference with those in T. repens in soil with Cu addition of 0 mg·kg^-1. The above results indicate that T. repens is adaptable to Cu-contaminated soil with a certain degree. All tested indexes of T. repens in inoculation group （successively inoculated with 2.7×109 and 2.3×109 CFU·mL^-1 suspensions） in soil with different Cu additions are basically higher than those of T. repens in the control group； in which， height and fresh and dry masses of above and underground parts of T. repens in inoculation group are basically significantly higher than those of T. repens in the control group in soil with Cu additions of 0， 500， and 1 000 mg·kg^-1. In addition， Cu content in above- and under-ground parts of T. repens in inoculation group is significantly higher than that of T. repens in the control group in soil with Cu additions of 500 and 1 000 mg·kg^-1. It is suggested that R. trifolii ACCC18017 can promote growth of T. repens and its absorption of Cu in soil， which is beneficial to remediation of Cu-contaminated soil with a certain degree.