Contents of rutin and catechin in leaves and under-ground part of Potentilla fragarioides L. in spring and autumn were determined by HPLC technology. The results show that in spring, rutin content in leaves and under-ground part of P. fragarioides is 1. 670 3 and 0. 054 4 mg·g^-1 , and catechin content is 0. 263 5 and 0. 723 5 mg·g^-1 , respectively. In autumn, rutin content is 0. 586 9 and 0. 010 7 mg·g^-1 , and catechin content is 0. 784 3 and 0. 366 4 mg·g^-1 , respectively. The rutin content in leaves is the highest in spring, and catechin content in leaves is the highest in autumn. Therefore, collecting leaves in spring is suitable for taking rutin as a purpose, while collecting leaves in autumn is suitable for taking catechin as a purpose.