研究了平板式光生物反应器中紫球藻(Porphyridium cruentum Naegeli)的培养条件,运用均匀设计法对光照强度、通气速率、装液量、接种密度以及pH等影响紫球藻生长的因素进行优化,获得了在平板式光生物反应器中培养紫球藻的最佳条件:光照强度10 000 lx、通气速率350 L·h^-1、装液量6 L、藻细胞接种密度1.1×106mL^-1、pH9.0。在最佳条件下藻体的生物量产率和生物量产量分别达到0.431 g·L^-1·d^-1和3.240 g·L^-1,最大生长速率达0.652 g·L^-1·d^-1,胞外多糖含量高达0.665 g·L^-1。另外,在培养过程中隔天补充培养液有利于紫球藻生物量的增加和胞外多糖的产生。

The effects of culture conditions on growth of Porphyridium cruentum Naegeli in the flat plate photobioreactor was investigated by means of the uniform. design method, which included light intensity, aeration rate, liquid volume, inoculation density and pH value. The optimal culture conditions were obtained as follows: light intensity 10 000 lx, aeration rate 350 L·h^-1, liquid volume 6 L, inoculation density 1. 1× 106 mL^-1 and pH 9. 0. Under the optimal culture conditions, the biomass production rate and biomass reached to 0. 431 g·L^-1·  d^-1 and 3. 240 g · L^-1 respectively, maximal growth rate was0. 652 g ·L^-1 ·d^-1 and exopolysaccharide content was 0.665 g·L^-1. In addition, the replenishment of fresh medium added every two days was beneficial to enhance the biomass production and exopolysaccharide content.