The volatile oil of Lysimachia foenum-graecum Hance grown in Jinxiou County of Guangxi Zhuangzu Autonomous Region was extracted with the steam distillation, the constituents and contents of the volatile oil were analyzed by means of GC-MS-DS technique. There are 130 components in the oil, in which 77 components have been identified. The major components were 9,12-octadecadienoic acid (10.265%), heptadecanoic acid (7.894%), phytol (5.277%), undecanal (3.566%), butyl phthalate (2.719%), etc.