阔鳞鳞毛蕨〔Dryopteris championi(Benth.)C.Chr.〕的叶片组织经硫酸铵沉淀、活性炭柱及DEAE Sepharose FF离子交换柱等步骤纯化得到鳞毛蕨凝集素(Dryopteris championi lectin)。纯化的鳞毛蕨凝集素(DCL)在聚丙烯酰胺凝胶电泳上显示1条蛋白质着色带。其中性糖含量高,氨基酸组成中Phe(苯丙氨酸)含量最高,His(组氨酸)含量最低。对不同动物红细胞及人的不同血型红细胞的凝集有专一性。其凝血活性能被果糖、半乳糖和N 乙酰半乳糖胺所抑制,对温度变化较不敏感,Mn²⁺和Mg²⁺在一定浓度范围能激活其为EDTA-Na₂所抑制的活性。

Dryopteris championi lectin was purified from the leaves of Dryopteris championi ( Benth. ) C . Chr. by precipitation with solid ammonium sulfate, decolorizing in active carbon column and further through DEAES epharose FF column. The purified lectin showed a single protein band on PAGE and has a high content of neutral saccharide. In its amino acid composition, Phe has the highest content and His has the lowest content. This lectin showed specific agglutination with different animal and human erythrocytes. The agglutinic activity of this lectin was insenstive to temperature change, but inhibited by three sugars ( Fru, Gal and GalNAc) . Inhibition of EDTA-Na₂ was activated with the presence of Mn²⁺ and Mg²⁺ at a certain concentration range.