摘要 | 以NH4Cl为氮源,设置0.0、0.5、2.5、5.0、10.0、20.0和40.0 mg·L-17个氮质量浓度水平,对培养21 d后各处理组凤眼蓝〔Eichhornia crassipes (Mart.) Solms〕的生长和生理指标尤其是氮代谢关键酶(包括谷氨酸脱氢酶、硝酸还原酶、亚硝酸还原酶、谷氨酰胺合成酶和谷氨酸合成酶)活性进行比较,对这些指标与氮质量浓度进行Pearson相关性分析,并对与氮质量浓度显著相关的指标和全氮含量进行Z分综合评价。结果表明:总体上看,随着氮质量浓度提高,株高、硝酸还原酶活性、亚硝酸还原酶活性、全氮含量和氮增加量先升高后降低,单株鲜质量、单株干质量、全磷含量和磷增加量先升高后趋于稳定,叶绿素相对含量、蛋白质含量和谷氨酰胺合成酶活性逐渐升高,根长逐渐下降,而谷氨酸脱氢酶活性和谷氨酸合成酶活性则波动变化。根长与氮质量浓度呈极显著(p<0.01)负相关,硝酸还原酶活性和亚硝酸还原酶活性与氮质量浓度呈显著(p<0.05)负相关,蛋白质含量与氮质量浓度呈极显著正相关,单株干质量、叶绿素相对含量和谷氨酰胺合成酶活性与氮质量浓度呈显著正相关。氮质量浓度5.0~40.0 mg·L-1处理组相关指标综合评价值的总和(∑Zi)均为正值,其中,氮质量浓度40.0 mg·L-1处理组的∑Zi值最高(4.91),氮质量浓度5.0和10.0 mg·L-1处理组的∑Zi值较高(分别为2.55和1.29)。综合所有结果,适合凤眼蓝生长发育的氮质量浓度为5.0~40.0 mg·L-1,尤其是5.0~10.0 mg·L-1,既能保证植株正常生长,又能充分发挥植株的氮吸收能力。 |
Abstract | Taking NH4Cl as the nitrogen source, seven mass concentration levels of nitrogen were set as 0.0, 0.5, 2.5, 5.0, 10.0, 20.0, and 40.0 mg·L-1, and growth and physiological indexes, especially key enzyme (containing glutamate dehydrogenase, nitrate reductase, nitrite reductase, glutamine synthetase, and glutamate synthetase) activities of nitrogen metabolism of each treatment group of Eichhornia crassipes (Mart.) Solms cultured for 21 d were compared, meanwhile, Pearson correlation analysis on these indexes and nitrogen mass concentrations was conducted, and Z-score comprehensive evaluations on indexes significantly correlated with nitrogen mass concentration and total nitrogen content were conducted. The results show that, in general, with the increase of nitrogen mass concentration, plant height, nitrate reductase activity, nitrite reductase activity, total nitrogen content, and nitrogen increment first increase and then decrease, fresh mass per plant, dry mass per plant, total phosphorus content, and phosphorus increment first increase and then tend to be stable, relative content of chlorophyll, protein content, and glutamine synthetase activity gradually increase, root length gradually decreases, while glutamate dehydrogenase activity and glutamate synthetase activity fluctuate. Root length shows an extremely significantly (p<0.01) negative correlation with mass concentration of nitrogen, nitrate reductase activity and nitrite reductase activity show significantly (p<0.05) negative correlations with nitrogen mass concentration, protein content shows an extremely significantly positive correlation with nitrogen mass concentration, and dry mass per plant, relative content of chlorophyll, and glutamine synthetase activity show significantly positive correlations with nitrogen mass concentration. The sum (∑Zi) of comprehensive evaluation values of relevant indexes of treatment groups treated with nitrogen mass concentrations of 5.0-40.0 mg·L-1 is positive, in which, the ∑Zi value of treatment group treated with nitrogen mass concentration of 40.0 mg·L-1 is the highest (4.91), and those of treatment groups treated with nitrogen mass concentrations of 5.0 and 10.0 mg·L-1 are relatively high (which are 2.55 and 1.29 respectively). Taken together, nitrogen mass concentrations suitable for growth and development of E. crassipes are 5.0-40.0 mg·L-1, especially 5.0-10.0 mg·L-1, which can both guarantee the normal growth of plant and fully exert nitrogen absorption ability of plant. |
关键词 | 凤眼蓝; 氮质量浓度; 氮代谢关键酶活性; 相关性分析; Z分综合评价 |
Key words | Eichhornia crassipes (Mart.) Solms; nitrogen mass concentration; key enzyme activity of nitrogen metabolism; correlation analysis; Zscore comprehensive evaluation |
作者 | 张迎颖1, 宋雪飞1, 徐佳兵2, 周庆1, 刘海琴1, 王岩1, 张志勇1, 宋海亮3 |
所在单位 | 1. 江苏省农业科学院农业资源与环境研究所 农业农村部长江下游平原农业环境重点实验室, 江苏 南京 210014;2. 常州市武进区前黄水利(务)站, 江苏 常州 213172; 3. 南京师范大学环境学院, 江苏 南京 210023 |
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基金项目 | 国家重大科技专项水体污染控制与治理专项(2017ZX07202004-07); 江苏省农业科技自主创新资金项目〔CX(20)2027〕; 江苏省自然科学基金项目(BK20181249) |