摘要 | 对同质园栽培的来自14个种源的三叶崖爬藤(Tetrastigma hemsleyanum Diels et Gilg)的10个表型性状进行比较,并进行聚类分析和相关性分析。结果表明:除新稍长和单株块茎数量外,三叶崖爬藤的茎粗、叶长、叶宽、单叶面积、块茎直径、单株块茎鲜质量以及块茎中总黄酮和多糖含量在种源间的差异达到极显著水平。三叶崖爬藤10个表型性状的变异系数在17.03%~51.95%之间,其中,块茎直径和单株块茎鲜质量的变异系数较大,分别为51.95%和48.59%。聚类分析结果显示:14个种源三叶崖爬藤聚为2类,其中,浙江6个种源聚为一类,该类三叶崖爬藤的茎较纤细,叶片较小,块茎中总黄酮和多糖含量较高;湖南、湖北、江西和广西种源聚为另一类,该类三叶崖爬藤的茎粗壮,叶片较大,块茎直径和单株块茎鲜质量较大。相关性分析结果显示:三叶崖爬藤块茎直径和单株块茎鲜质量与茎粗、新稍长、叶宽和单叶面积呈极显著正相关,块茎中总黄酮和多糖含量与茎粗、新稍长、叶长、叶宽、单叶面积、块茎直径和单株块茎鲜质量总体上呈极显著负相关。研究结果显示:三叶崖爬藤表型性状可作为种源划分和优良种源筛选的依据。 |
Abstract | Ten phenotypic characters of Tetrastigma hemsleyanum Diels et Gilg from fourteen provenances cultivated in the common garden were compared, and cluster and correlation analyses were also conducted. The results show that except for new shoot length and tuber number per plant, there are extremely significant differences in stem diameter, leaf length, leaf width, single leaf area, tuber diameter, tuber fresh mass per plant, and total flavone and polysaccharide contents in tuber of T. hemsleyanum among provenances. Coefficient of variation of ten phenotypic characters of T. hemsleyanum is in the range of 17.03%-51.95%, in which, that of tuber diameter and tuber fresh mass per plant is relatively big, with values of 51.95% and 48.59%, respectively. The cluster analysis result shows that T. hemsleyanum from fourteen provenances can be clustered into two categories, in which, six provenances from Zhejiang are clustered into one category, which have relatively slender stem and small leaf but relatively high contents of total flavone and polysaccharide in tuber; provenances from Hunan, Hubei, Jiangxi, and Guangxi are clustered into the other category, which have strong stem, relatively big leaf, and relatively large tuber diameter and tuber fresh mass per plant. The result of correlation analysis shows that there are extremely significant positive correlations of tuber diameter and tuber fresh mass per plant of T. hemsleyanum with stem diameter, new shoot length, leaf width, and single leaf area, and in general, there are extremely negative correlations of total flavone and polysaccharide contents in tuber with stem diameter, new shoot length, leaf length, leaf width, single leaf area, tuber diameter, and tuber fresh mass per plant. It is suggested that phenotypic characters of T. hemsleyanum can be used as the criteria for provenance division and screening of superior provenance. |
关键词 | 三叶崖爬藤; 同质园栽培; 表型多样性; 变异系数; 聚类分析; 相关性分析 |
Key words | Tetrastigma hemsleyanum Diels et Gilg; common garden cultivation; phenotypic diversity; coefficient of variation; cluster analysis; correlation analysis |
作者 | 杨旭1,2, 杨志玲1,2, 程小燕1,2, 谭美1,2 |
所在单位 | 1. 中国林业科学研究院亚热带林业研究所, 浙江 杭州 311400; 2. 浙江省林木育种技术研究重点实验室, 浙江 杭州 311400 |
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下载次数 | 1058 |
基金项目 | 浙江省公益技术研究农业项目(2016C32029); 浙江省农业新品种选育重大科技专项(2016C02058-4) |