2022年1月21日 星期五
芹菜品种‘津南实芹’AgICE1基因的 克隆及其对非生物胁迫的响应
Cloning of AgICE1 gene from cultivar ‘Jinnan Shiqin’ of Apium graveolens and its response to abiotic stresses
2019年 第28卷 第1期 页码[16-24]    下载全文[3.4MB]  
摘要

 从芹菜(Apium graveolens Linn.)品种‘津南实芹’(‘Jinnan Shiqin’)叶片RNA中克隆获得1个转录因子基因,命名为AgICE1。序列分析结果显示:AgICE1基因含有1个长度为1 455 bp的开放阅读框(ORF),编码484个氨基酸。AgICE1转录因子氨基酸序列的理论相对分子质量为53 140,理论等电点为pI 5.09,为亲水性蛋白。AgICE1转录因子的氨基酸组成中脂肪族氨基酸的比例最高,酸性氨基酸的比例次之,碱性氨基酸的比例较低,芳香族氨基酸的比例最低。序列比对与系统进化分析结果表明:ICE1转录因子在不同植物中高度保守。实时荧光定量PCR检测结果表明:AgICE1基因在叶片中的相对表达水平显著高于在根和叶柄中的相对表达水平;在高温(38 ℃)、低温(4 ℃)、干旱(质量体积分数20% PEG6000)和高盐(0.2 mol·L-1 NaCl)处理下,AgICE1基因的相对表达水平总体上升高。研究结果表明:AgICE1转录因子在芹菜非生物胁迫调控中起重要作用。

Abstract

A transcription factor gene was cloned from RNA in leaf blade of cultivar ‘Jinnan Shiqin’ of Apium graveolens Linn., which was named AgICE1. Sequence analysis result shows that there is an open reading frame. (ORF) in AgICE1 gene with length of 1 455 bp, which encodes 484 amino acids. Theoretical relative molecular mass of amino acid sequence of transcription factor AgICE1 is 53 140, theoretical isoelectric point is pI 5.09, and amino acid sequence of transcription factor AgICE1 is a hydrophilic protein. The percentage of aliphatic amino acid is the highest in amino acid composition of transcription factor AgICE1, that of acidic amino acid comes second, that of basic amino acid is relatively low, and that of aromatic amino acid is the lowest. Sequence alignment and phylogenetic analysis results show that transcription factor ICE1 is highly conserved in different species. Real-time fluorescence quantitative PCR determination result shows that relative expression level of AgICE1 gene in leaf blade is significantly higher than that in root and petiole, and its relative expression level increases in general in treatments of high temperature (38 ℃), low temperature (4 ℃), drought (mass volume fraction of 20% PEG6000), and high salt (0.2 mol·L-1 NaCl). It is suggested that transcription factor AgICE1 plays an important role in regulation of abiotic stresses of A. graveolens.

关键词芹菜; AgICE1转录因子; 基因克隆; 非生物胁迫; 相对表达水平
Key wordsApium graveolens Linn; transcription factor AgICE1; gene cloning; abiotic stress; relative expression level
作者杨青青, 刘洁霞, 徐志胜, 冯凯, 黄莹, 熊爱生
所在单位南京农业大学园艺学院 作物遗传与种质创新国家重点实验室 农业部华东地区园艺作物生物学与种质创制重点实验室, 江苏 南京 210095
点击量1227
下载次数323
基金项目江苏省农业科技自主创新资金项目(CX18-2007); 国家自然科学基金资助项目(31272175); 国家教育部新世纪优秀人才支持计划项目(NCET-11-0670); 江苏高校优势学科建设项目(PAPD)