2019年10月17日 星期四
氮素形态及配比对夏播菘蓝生长及活性成分含量的影响
Effects of nitrogen form and ratio on growth and active component content in summer-planted Isatis indigotica
2017年 第26卷 第1期 页码[21-29]    下载全文[1.1MB]  
摘要

以来自山西的菘蓝(Isatis indigotica Fort.)为实验对象,采用盆栽法研究铵态氮(NH4+-N)、硝态氮(NO3--N)和酰胺态氮〔CO(NH22〕的不同配比对夏播菘蓝生长,叶和根中的可溶性蛋白质及总氮含量,根中多糖含量,叶中叶绿素相对含量,以及叶中靛玉红和靛蓝、根中(R,S)-告依春的含量和积累量的影响。结果表明:各施氮处理组的单株叶干质量均高于对照(不施用氮素)组,但单株根干质量或高于或低于对照组,其中,T4〔n(铵态氮)∶n(硝态氮)∶n(酰胺态氮)=25∶75∶0〕处理组的单株叶和根干质量均最大,且总体上显著高于对照组及其他施氮处理组(P<0.05);而施氮处理组的根冠比均显著低于对照组。各施氮处理组叶中的可溶性蛋白质含量与对照均无显著差异,但各施氮处理组根中的可溶性蛋白质含量、叶和根中的总氮含量以及叶中的叶绿素相对含量总体上显著高于对照组,而根中的多糖含量或高于或低于对照组,其中,T6〔n(铵态氮)∶n(硝态氮)∶n(酰胺态氮)=0∶75∶25〕处理组根中的多糖含量和叶中的叶绿素相对含量均最高,T3〔n(铵态氮)∶n(硝态氮)∶n(酰胺态氮)=50∶50∶0〕处理组叶和根中的可溶性蛋白质含量均较高。各施氮处理组叶中靛玉红含量总体上显著高于对照组,多数施氮处理组叶中靛蓝含量则显著低于对照组,但各施氮处理组的单株叶中靛蓝和靛玉红积累量总体上高于对照组;其中,T2〔n(铵态氮)∶n(硝态氮)∶n(酰胺态氮)=75∶25∶0〕处理组叶中靛玉红含量及其单株积累量均最高,T6处理组叶中靛蓝含量最高,而单株叶中靛蓝积累量则以T3处理组最高。各施氮处理组根中(R,S)-告依春含量总体上显著低于对照组,其中,以T1〔n(铵态氮)∶n(硝态氮)∶n(酰胺态氮)=100∶0∶0〕处理组根中(R,S)-告依春含量最高,T4处理组单株根中(R,S)-告依春积累量最高。综合分析结果表明:按不同配比施用不同形态氮素,夏播菘蓝的生长及活性成分含量有明显差异,因此,若以收获叶为目的,结合叶中靛玉红含量,建议施用铵态氮和硝态氮物质的量比为75∶25的复合氮肥;若以收获根为目的,结合根中(R,S)-告依春含量,建议施用铵态氮和硝态氮物质的量比为25∶75的复合氮肥。

 

Abstract

Taking Isatis indigotica Fort. from Shanxi as experimental object, effects of ammonium nitrogen (NH4+-N), nitrate nitrogen (NO3--N) and amide nitrogen 〔CO(NH22〕 with different ratios on seedling growth, contents of soluble protein and total nitrogen in leaf and root, polysaccharide content in root, relative chlorophyll content in leaf, contents and accumulations of indigo and indirubin in leaf and (R,S)-epigoitrin in root of summer-planted I. indigotica were investigated by pot culture method. The results show that leaf dry weight per plant in all nitrogen treatment groups is higher than that in the control (without applying nitrogen) group, but root dry weight per plant is higher or lower than that in the control group. In which, in T4 〔n(ammonium nitrogen)∶n(nitrate nitrogen)∶n(amide nitrogen)〕=25∶75∶0〕 treatment group, dry weights of leaf and root per plant are the highest, and are generally significantly higher than those in the control group and other nitrogen treatment groups (P<0-05). While root/shoot ratio in all nitrogen treatment groups is significantly lower than that in the control group. There is no significant difference of soluble protein content in leaf between all nitrogen treatment groups and the control group, but soluble protein content in root, total nitrogen content in leaf and root and relative chlorophyll content in leaf in all nitrogen treatment groups are generally significantly higher than those in the control group, while polysaccharide content in root is higher or lower than that in the control group. In which, in T6 〔n(ammonium nitrogen)∶n(nitrate nitrogen)∶n(amide nitrogen)〕=0∶75∶25〕 treatment group, polysaccharide content in root and relative chlorophyll content in leaf are the highest, and in T3 〔n(ammonium nitrogen)∶n(nitrate nitrogen)∶n(amide nitrogen)〕=50∶50∶0〕 treatment group, soluble protein content in leaf and root is relatively high. Indirubin content in leaf in all nitrogen treatment groups is generally significantly higher than that in the control group, indigo content in leaf in most nitrogen treatment groups is significantly lower than that in the control group, while accumulations of indirubin and indigo in leaf per plant in all nitrogen treatment groups are generally higher than those in the control group. In which, content and accumulation per plant of indirubin in leaf in T2 〔n(ammonium nitrogen)∶n(nitrate nitrogen)∶n(amide nitrogen)〕=75∶25∶0〕 treatment group are the highest, indigo content in leaf in T6 treatment group is the highest, while indigo accumulation in leaf per plant in T3 treatment group is the highest. (R,S)-epigoitrin content in root in all nitrogen treatment groups is generally significantly lower than that in the control group, in which, that in T1〔n(ammonium nitrogen)∶n(nitrate nitrogen)∶n(amide nitrogen)〕=100∶0∶0〕 treatment group is the highest, and (R,S)-epigoitrin accumulation in root per plant in T4 treatment group is the highest. The comprehensive analysis result shows that there are obvious differences in growth and active component content in summer-planted I. indigotica by appling nitrogen with different ratios and forms. Therefore, it is suggested that combined indirubin content in leaf, ammonium nitrogen and nitrate nitrogen with molar ratio of 75∶25 can be used as compound nitrogen fertilizer for the purpose of harvesting leaf, and combined (R,S)-epigoitrin content in root, ammonium nitrogen and nitrate nitrogen with molar ratio of 25∶75 can be used as compound nitrogen fertilizer for purpose of harvesting root.
 

关键词夏播菘蓝; 氮素形态; 氮素比例; 生长性状; 靛玉红含量; (R,S)-告依春含量
Key wordssummer-planted Isatis indigotica Fort.; nitrogen form; nitrogen ratio; growth trait; indirubin content; (R,S)-epigoitrin content
作者唐晓清, 杨月, 吕婷婷, 张毅, 李艳, 王康才
所在单位南京农业大学中药材研究所, 江苏 南京 210095
点击量424
基金项目国家自然科学基金资助项目(31171486); 国家级大学生创新创业训练计划项目(201310307026)