采用盆栽法研究了质量浓度0. 0(对照)、50. 0、25. 0、13. 3 和12. 5 mg·L^-15-氨基乙酰丙酸(ALA)处理1 至3 次(每次间隔10 d)对菘蓝(Isatis indigotica Linn.)苗期生长的影响;并采用高效液相色谱法分析了叶片内靛蓝和靛玉红含量的变化。结果表明:处理1 至3 次后各处理组菘蓝根的干质量均高于对照且总体上差异极显著,其中,以12. 5 mg·L^-1 ALA 处理组根干质量最高;多数处理组根鲜质量也高于对照,其中用12. 5 mg·L^-1 ALA 处理3 次根的鲜质量最高。处理1 次或2 次后各处理组叶片干质量和鲜质量或高于对照或低于对照,其中12. 5 mg·L^-1采用盆栽法研究了质量浓度0. 0(对照)、50. 0、25. 0、13. 3 和12. 5 mg·L^-15-氨基乙酰丙酸(ALA)处理1 至3 次(每次间隔10 d)对菘蓝(Isatis indigotica Linn.)苗期生长的影响;并采用高效液相色谱法分析了叶片内靛蓝和靛玉红含量的变化。结果表明:处理1 至3采用盆栽法研究了质量浓度0. 0(对照)、50. 0、25. 0、13. 3 和12. 5 mg·L^-15-氨基乙酰丙酸(ALA)处理1 至3 次(每次间隔10 d)对菘蓝(Isatis indigotica Linn.)苗期生长的影响;并采用高效液相色谱法分析了叶片内靛蓝和靛玉红含量的变化。结果表明:处理1 至3 次后各处理组菘蓝根的干质量均高于对照且总体上差异极显著,其中,以12. 5 mg·L^-1 ALA 处理组根干质量最高;多数处理组根鲜质量也高于对照,其中用12. 5 mg·L^-1 ALA 处理3 次根的鲜质量最高。处理1 次或2 次后各处理组叶片干质量和鲜质量或高于对照或低于对照,其中12. 5 mg·L^-1   ALA 处理组叶片的干质量和鲜质量均极显著高于对照;但处理3 次后各处理组的叶片干质量和鲜质量也均极显著高于对照。处理1 次或2 次后各处理组的根冠比总体上高于对照,而处理3 次后各处理组的根冠比均低于对照。处理1 次或2 次后各处理组叶片中靛蓝和靛玉红含量均高于对照,且多数处理组靛蓝和靛玉红含量与对照有显著或极显著差异;而处理3 次后各处理组的靛蓝含量均显著高于对照,但仅13. 3 和12. 5 mg·L^-1 ALA 处理组靛玉红含量极显著高于对照;总体上看,用13. 3 和12. 5 mg·L^-1 ALA 处理2 次或3 次,叶片中靛蓝和靛玉红含量均显著或极显著高于对照, 其中, 用12. 5 mg·L^-1 ALA 处理3 次靛蓝含量最高(22. 51 μg·g^-1 ), 用13. 3 mg·L^-1 ALA 处理3 次靛玉红含量最高(99. 48 μg·g^-1 )。研究结果表明:采取适宜的ALA 处理对菘蓝生长及叶片中有效成分积累均有一定的促进作用,其中,用低浓度ALA 喷施2 或3 次较为适宜。

Effect of 0. 0 (CK), 50. 0, 25. 0, 13. 3 and 12. 5 mg·L-1 5-aminolevulinic acid (ALA) treating for one to three times at intervals of 10 d on seedling growth of Isatis indigotica Linn. was studied by pot method, and content change of indigo and indirubin in leaf was analyzed by HPLC. The results show that after treating for one to three times, root dry weight of all ALA treatment groups is higher than that of the control with the extremely significant difference generally, in which, that of 12. 5 mg·L^-1 ALA treatment group is the highest; root fresh weight in most of treatment groups is also higher than that of the control, in which, that of the group treated by 12. 5 mg·L^-1 ALA for three times is the highest. After treating for one or two times, dry weight and fresh weight of leaf in all ALA treatment groups are higher or lower than those of the control, in which, those of 12. 5 mg·L-1 ALA treatment group are very significantly higher than those of the control; but after treating for three times, dry weight and fresh weight of leaf in all ALA treatment groups are all very significantly higher than those of the control. After treating for one or two times, root-shoot ratio in all ALA treatment groups is generally higher than that of the control, but that after treating for three times is lower than that of the control. After treating for one or two times, contents of indigo and indirubin in all ALA treatment groups are higher than those of the control and there are significant or extremely significant differences in their contents between the control and most of ALA treatment groups. While after treating for three times, indigo content in all ALA treatment groups is significantly higher than that of the control, but indirubin content only in 13. 3 and 12. 5 mg·L^-1 ALA treatment groups is extremely significantly higher than that of the control. Generally, contents of indigo and indirubin after treating by 13. 3 and 12. 5 mg·L^-1 ALA for two or three times are significantly or extremely significantly higher than those of the control, in which, indigo content in the group treated by 12. 5 mg·L^-1 ALA for three times is the highest (22. 51 μg·g^-1 ), and indirubin content in the group treated by 13. 3 mg·L^-1 ALA for three times is the highest (99. 48 μg·g^-1). It is suggested that suitable ALA treatment has a promoting role to growth and accumulation of active components of I. indigotica, in which, spraying for two or three times with low concentration of ALA isappropriate.