2021年4月23日 星期五
沙棘叶片、果肉和种子中黄酮类成分的差异
Difference in flavonoid constituents in leaf, pulp, and seed of Hippophae rhamnoides
2019年 第28卷 第4期 页码[58-67]    下载全文[1MB]  
摘要

以46个蒙古沙棘(Hippophae rhamnoides subsp. mongolica Rousi)品种(品系、优系)和6个中国沙棘与蒙古沙棘的杂交品系(优系)(H. rhamnoides subsp. sinensis×subsp. mongolica)为研究样本,采用优化的LC-MS/MS法对各样本叶片、果肉和种子中黄酮类成分进行检测,并对52个样本不同器官中黄酮类成分含量的差异进行比较和分析。结果显示:异鼠李素、槲皮素、山柰酚、表没食子儿茶素、芦丁、没食子儿茶素没食子酸酯、木犀草素和柚皮苷8个黄酮类成分在供试样本叶片中均可检出,但果肉和种子中黄酮类成分组成存在差异;除山柰酚外,其余7个成分均可从果肉中检出,但部分样本果肉仅含其中的3~6个成分,且大多数样本果肉不含没食子儿茶素没食子酸酯;从种子中虽然检出8个黄酮类成分,但所有样本种子仅含有其中的3~7个成分,且大多数样本种子不含山柰酚和木犀草素。供试52个样本叶片中芦丁、槲皮素、表没食子儿茶素、山柰酚和异鼠李素的含量较高,分别为33.806~195.783、24.725~161.888、4.611~110.501、3.640~13.508和1.301~26.923 μg·g-1;果肉中芦丁、槲皮素、表没食子儿茶素和异鼠李素的含量较高,分别为12.510~136.558、5.451~19.932、1.688~3.738和0.160~9.601 μg·g-1;种子中表没食子儿茶素、芦丁、槲皮素和异鼠李素的含量较高,分别为4.661~31.488、3.306~55.204、5.121~15.149和0.386~11.175 μg·g-1;叶片、果肉和种子中黄酮类成分的总含量分别为105.384~341.698、16.294~152.129和8.771~73.360 μg·g-1,以叶片中黄酮类成分的总含量最高。供试52个样本不同器官中8个黄酮类成分含量存在不同程度的变异,变异系数为1.421%~101.211%。蒙古沙棘品种(品系、优系)叶片、果肉和种子中黄酮类成分的总含量之和显著高于中国沙棘与蒙古沙棘的杂交品系(优系)。综合分析结果表明:供试52个沙棘品种(品系、优系)叶片、果肉和种子中黄酮类成分的组成和含量均存在明显差异,但主要黄酮类成分均为芦丁、槲皮素和表没食子儿茶素。建议在实际生产中,依据沙棘黄酮的应用目标对沙棘品种(品系、优系)进行定向选育。

Abstract

Taking 46 cultivars (strains, superior lines) of Hippophae rhamnoides subsp. mongolica Rousi and 6 hybrid strains (superior lines) of H. rhamnoides subsp. sinensis×subsp. mongolica as research samples, flavonoid constituents in leaf, pulp, and seed of each sample were detected by optimized LC-MS/MS method, and the differences in contents of flavonoid constituents in different organs of 52 samples were compared and analyzed. The results show that 8 flavonoid constituents of isorhamnetin, quercetin, kaempferol, (-)-epigallocatechin, rutin, (-)-gallocatechingallate, luteolin, and naringin can be detected in leaf of all test samples, but the composition of flavonoid constituents is different in pulp and seed; except kaempferol, other 7 flavonoid constituents can be detected in pulp, but pulp of some samples only contains 3-6 constituents of them, and pulp of most samples does not contain (-)-gallocatechingallate; although 8 flavonoid constituents can be detected in seed, but seed of all samples only contains 3-7 constituents of them, and seed of most samples does not contain kaempferol and luteolin. Contents of rutin, quercetin, (-)-epigallocatechin, kaempferol, and isorhamnetin in leaf of 52 test samples are relatively high, which are 33.806-195.783, 24.725-161.888, 4.611-110.501, 3.640-13.508, and 1.301-26.923 μg·g-1, respectively; contents of rutin, quercetin, (-)-epigallocatechin, and isorhamnetin in pulp are relatively high, which are 12.510-136.558, 5.451-19.932, 1.688-3.738, and 0.160-9.601 μg·g-1, respectively; contents of (-)-epigallocatechin, rutin, quercetin, and isorhamnetin in seed are relatively high, which are 4.661-31.488, 3.306-55.204, 5.121-15.149, and 0.386-11.175 μg·g-1, respectively; total contents of flavonoid constituents in leaf, pulp, and seed are 105.384-341.698, 16.294-152.129, and 8.771-73.360 μg·g-1, respectively, and its total content in leaf is the highest. There are different degrees of variation in contents of 8 flavonoid constituents among different organs of 52 test samples with the coefficients of variation of 1.421%-101.211%. The sum of total contents of flavonoid constituents in leaf, pulp, and seed of cultivars (strains, superior lines) of H. rhamnoides subsp. mongolica is significantly higher than that of hybrid strains (superior lines) of H. rhamnoides subsp. sinensis×subsp. mongolica. The comprehensive analysis result shows that there are obvious differences in composition and contents of flavonoid constituents in leaf, pulp, and seed of 52 test H. rhamnoides cultivars (strains, superior lines), but the main flavonoid constituents are all rutin, quercetin, and (-)-epigallocatechin. It is suggested that directive breeding of H. rhamnoides cultivars (strains, superior lines) should be performed according to the application purpose of flavonoid constituents in practical production.

关键词蒙古沙棘; 中国沙棘; 种质资源; LC-MS/MS; 黄酮类成分
Key wordsHippophae rhamnoides subsp. mongolica Rousi; H. rhamnoides subsp. sinensis Rousi; germplasm resources; LC-MS/MS; flavonoid constituents
作者蔡爽a,b, 阮成江a,b, 杜维a,b, 丁健a,b, 韩平a,b, 王海明a,b
所在单位大连民族大学: a. 资源植物研究所, b. 生物技术与资源利用教育部重点实验室, 辽宁 大连 116600
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基金项目国家自然科学基金资助项目(31570681); 欧盟第七框架计划玛丽居里行动国际人才引进奖学金回国阶段项目(PIIFR-GA-2010-910048)