对羌活（Notopterygium incisum Ting ex H. T. Chang）种子进行层积处理，观测层积过程中种子种胚形态及胚率，研究种子中营养物质和内源激素含量以及呼吸途径关键酶活性的变化；并对层积后的种子进行氟啶酮处理，测定种子的发芽率和发芽势。结果显示：与未层积（层积0 d）相比，层积后羌活种子种胚由球形发育为子叶型，胚率增长至67.30%，发芽率增长至50.67%；种子的蛋白质、可溶性糖和淀粉含量显著降低；赤霉素（GA3）和吲哚乙酸（IAA）含量分别在59.15~81.06和14.06～22.08 ng·g-1范围内波动变化，脱落酸（ABA）含量在层积150和180 d时显著降低，GA3/ABA比和（GA3+IAA）/ABA比在层积120~180 d显著升高。在层积0～60 d，羌活种子的丙酮酸激酶（PK）活性呈先升高后降低的趋势，琥珀酸脱氢酶（SDH）活性明显升高，葡萄糖-6-磷酸脱氢酶（G-6-PDH）活性先降低后略有升高，该时期以三羧酸循环途径（TCA）为主；在层积60～150 d，PK活性呈波动升高的趋势，SDH和G-6-PDH活性总体呈波动降低的趋势，该期间以糖酵解途径（EMP）为主；在层积180 d时，PK、SDH和G-6-PDH活性均显著高于层积120 d。相关性分析结果显示：ABA含量分别与胚率和发芽率呈显著和极显著负相关，相关系数分别为-0.800和-0.875。综合研究结果表明：羌活种子具有胚后熟特性，层积处理结合氟啶酮处理可打破羌活种子休眠，提高种子的发芽率；营养物质含量的降低，呼吸途径关键酶活性的波动变化，ABA含量的降低以及GA3/ABA比和（GA3+IAA）/ABA比的升高对羌活种子休眠解除有重要影响。

Stratification treatment was conducted for Notopterygium incisum Ting ex H. T. Chang seeds, the embryo morphology and embryo rate of seeds during stratification were observed, and the variations of contents of nutrients and endogenous hormones and activities of key enzymes in respiratory pathway of seeds were studied; the seeds after stratification were treated with fluridone, and their germination rate and germination potential were measured. The results show that compared with no stratification (stratification for 0 d), the embryos of N. incisum seeds develop from spherical into cotyledon type after stratification, the embryo rate increases to 67.30%, and the germination rates increases to 50.67%; contents of protein, soluble sugar, and starch in seeds significantly decrease; contents of gibberellin (GA₃) and indoleacetic acid (IAA) fluctuate in the ranges of 59.15-81.06 and 14.06-22.08 ng·g-1 respectively, abscisic acid (ABA) content significantly decreases at stratification for 150 and 180 d, and GA₃/ABA and (GA₃+IAA)/ABA ratios significantly increase during stratification for 120-180 d. During stratification for 0-60 d, pyruvate kinase (PK) activity of N. incisum seeds shows a tendency to first increase and then decrease, succinic dehydrogenase (SDH) activity evidently increases, glucose-6-phosphate dehydrogenase (G-6-PDH) activity first decreases and then slightly increases, and tricarboxylic acid cycle pathway (TCA) is dominant in this stage; during stratification for 60-150 d, PK activity shows a fluctuation increase tendency, activities of SDH and G-6-PDH show a fluctuation decrease tendency in general, and embden meyerhof pathway (EMP) is dominant in this stage; at stratification for 180 d, activities of PK, SDH, and G-6-PDH are all significantly higher than those at stratification for 120 d. The correlation analysis result shows that ABA content shows a significant and an extremely significant negative correlation with embryo rate and germination rate, and the coefficients of correlation are -0.800 and -0.875 respectively. The comprehensive study result shows that N. incisum seeds have the embryo post-maturation characteristics, and stratification treatment combined with fluridone treatment can break the dormancy of N. incisum seeds and increase germination rate of seeds; the decrease of nutrient content, fluctuation variations of activities of key enzymes in respiratory pathway, decrease of ABA content, and increases of GA₃/ABA and (GA₃+IAA)/ABA ratios have important effects on the dormancy release of N. incisum seeds.