以湿地松( Pinus elliottii Engelm .) 的未成熟合子胚为外植体, 在附加 8 mg/L 2, 4-D 和 4mg/ L BA 的 LP 培养基上诱导出胚性愈伤组织。 在含 1 mg/ L 2, 4-D 和 0.5 mg/ L BA 的培养基上保持并增殖。 提高培养基的渗透压后, 愈伤组织内形成大量的胚性胚柄细胞团和早期原胚。 在附加 4 mg/ L ABA, 75 g/ L PEG 和 5 g/ L 活性炭的 LP 培养基上, 早期原胚发育形成粗壮的子叶胚。在无激素 LP 培养基上, 成熟的体细胞胚萌发并发育形成再生完整植株。 体细胞胚转换成小植株的频率为 15.6 %。
 

Embryogenic callus was initiated from immature zygotic embryos of slash pine ( Pinus elliottii Engelm .) cultured on LP medium containing 8 mg/ L 2, 4-D and 4 mg/L BA .Multiplication can be achieved on LP medium with 1 mg/ L 2, 4-D and 0 .5 mg/L BA . Embryonal suspensor masses and early stage proembryos were formed from embryogenic callus cultured on high osmo tic pressure medium .Early stage embry os developed into cotyledonary embryos on LP medium supplemented with 4 mg/ L ABA, 75 g/L PEG and 5 g/L activated charcoal.Mature somatic embryos germinated into regeneration plantlet on medium w ith hormone-free .The frequency of somatic embryos converted into regeneration plantlets is 15 .6 %