通过单因子和正交实验探讨了培养液中不同浓度KNO₃、Na₂HPO₄、FeCl₃和Na₂S iO₃对通过“神舟5号”飞船搭载与非搭载舟形藻(Navicula tenera)生长的影响,确立了适合搭载与非搭载舟形藻生长的最佳培养基组合。结果表明,经搭载后舟形藻生长所需的营养盐组合发生了变化。通过正交实验筛选出适合搭载舟形藻生长的最佳培养基为含300 mg·L^-1KNO3、60 mg·L^-1Na₂HPO₄·12H₂O、24 mg·L^-1FeCl₃和700 mg·L^-1Na₂SiO₃·9H₂O的F/2培养基;适合非搭载舟形藻生长的最佳培养基为含600 mg·L^-1KNO₃、60 mg·L^-1Na₂HPO₄·12H₂O、12mg·L^-1FeCl₃和700 mg·L^-1Na₂SiO₃·9H₂O的F/2培养基。 

The optimal combinations of different concentrations of KNO₃, Na₂HPO₄, FeCl₃andNa₂SiO₃ in culture media for spaceflight and nonspaceflight Navicula tenera were studied by single factor experiment and orthogonal experiment〔 L16(45)〕. The results indicated that the combinations and concentrations of nutrients in culture medium for N. tenera growth were changed after spaceflight. The best medium for growth of spaceflight N. tenera was F /2 containing 300 mg · L^-1 KNO₃, 60 mg·L^-1Na₂HPO₄·12H₂O, 24 mg·L^-1 FeCl₃and 700 mg·L^-1 Na₂SiO₃·9H₂O, while the best medium for nonspaceflight N. tenera was F/2 containing 600mg·L^-1 KNO₃, 60mg·L^-1 Na₂HPO₄ ·12H₂O, 12mg·L^-1 FeCl₃and 700 mg·L^-1 Na₂SiO₃·9H₂O.