摘要 | 以1/4 Hoagland溶液为基础培养液,研究了0.5%、1.0%、1.5%、2.0%和2.5%NaC l处理对海滨锦葵〔Kostelezkya virginica(L.)Presl.〕种子萌发和幼苗生长的影响,发现随着培养液中NaCl浓度的增加,海滨锦葵种子萌发率逐渐降低;当NaCl浓度达2.5%时种子不萌发,但NaCl胁迫解除后,种子的萌发率水平与对照相当。幼苗在含0.5%~1.0%NaCl的培养液中生长状况良好,叶绿素含量和根系活力明显增高;但当NaCl浓度达1.5%~2.0%时,叶绿素含量和根系活力逐渐下降;与对照相比,NaCl胁迫下幼苗的MDA水平降低。结果表明,NaCl胁迫对海滨锦葵种子萌发和幼苗生长有一定的影响,但海滨锦葵可通过种子休眠、增加根系活力、降低体内MDA水平来缓解一定的盐害效应,以适应盐胁迫的生长环境。 |
Abstract | Using 1 /4 Hoagland as the basic culture solution, the status of seed germination and seedling growth of Kostelezkya virginica(L.)Presl .under0. 5% -2. 5% NaCl stress was researched. With the increasing of NaCl concentration, the seed germination rate decreased gradually. The seeds treated by2. 5% NaCl did not germinated, but seeds still germinated and got similar germination rate as CK when NaCl stress was canceled. The seedlings growing in 0.5%-1.0%NaClculture solution were much better than that in higher salt concentration, and had higher chlorophyll content and root system activity than CK, but got bad and chlorophyll content was reduced when seedlings cultured in 1. 5% -2. 0% NaCl solution. The MDA content of seedling was decreased under NaCl stress. In addition, the adaptation mechanism of K. virginica to NaCl stress was discussed. |
关键词 | 海滨锦葵; 种子萌发; 盐胁迫; 生理特性; |
Key words | Kostelezkya virginica (L.) Pres.l; seed germination; salt stress; physiological characteristics |
作者 | 尹增芳1,何祯祥2,王丽霞1,钦 佩2 |
所在单位 | 1.南京林业大学森林资源与环境学院,江苏南京210037; 2.南京大学生命科学学院,江苏南京210093 |
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基金项目 | 国家高技术研究发展计划(“863”计划)资助项目(2002AA629210); |