基于扭果花旗杆（Dontostemon elegans Maxim.）转录组数据，鉴定其bHLH转录因子家族成员，并通过生物信息学方法分析该转录因子家族成员的理化性质、保守基序及结构域、系统进化关系、组织表达模式及逆境胁迫表达模式。结果表明：从扭果花旗杆转录组数据库中共筛选出66个bHLH转录因子家族成员，其氨基酸残基数为63～668，理论等电点为pI 4.74至pI 10.17，理论相对分子质量为7 396.56～73 508.47，65个家族成员属于亲水蛋白，60个家族成员定位在细胞核中；所有家族成员含有motif1和（或）motif2，且都含有保守的bHLH_SF超家族结构域。扭果花旗杆bHLH转录因子家族成员可划分为13个亚家族，亚家族含有的扭果花旗杆bHLH家族成员最多（10个）。组织表达模式分析结果表明：扭果花旗杆bHLH基因在不同组织中的相对表达量有所差异，De11684.2214、De11684.23639和De11684.33169在叶中的相对表达量最高，De11684.2906和De11684.31669在茎中的相对表达量最高，De11684.7549在根中的相对表达量最高，De11684.7900在根和叶中的相对表达量接近且显著（P<0.05)高于茎。扭果花旗杆bHLH基因对不同逆境胁迫有不同程度的响应：在150 mmol·L^-1 NaCl胁迫下，7个bHLH基因的相对表达量在不同时间均显著上调；在250 mmol·L^-1聚乙二醇（PEG）胁迫下，6个bHLH基因的相对表达量在2 h时显著下调；在4 ℃胁迫下，6个bHLH基因的相对表达量在8 h时显著上调。综合分析表明：扭果花旗杆bHLH转录因子家族成员具有bHLH转录因子家族的基本特征；bHLH基因具有组织表达特异性，bHLH家族成员可能参与盐胁迫和低温胁迫的调控过程。

 Based on the transcriptome data of Dontostemon elegans Maxim., its bHLH transcription factor family members were identified, and the physicochemical properties, conserved motifs and domains, phylogenetic relationships, tissue expression patterns, and adversity stress expression patterns of these transcription factor family members were analyzed by using bioinformatics methods. The results show that a total of 66 bHLH transcription factor family members are screened from the transcriptome database of D. elegans, the numbers of amino acid residues are 63-668, the theoretical isoelectric points are pI 4.74 to pI 10.17, the theoretical relative molecular masses are 7 396.56-73 508.47, 65 family members are hydrophilic proteins, and 60 family members are localized in nucleus; all family members contain motif1 and (or) motif2, and all contain conserved bHLH_SF superfamily domain. The bHLH transcription factor family members in D. elegans can be divided into 13 subfamilies, and subfamily - contains the most bHLH family members in D. elegans (10). The tissue expression pattern analysis result shows that the relative expressions of bHLH genes in D.elegans are different among different tissues, the relative expressions of De11684.2214, De11684.23639, and De11684.33169 are the highest in leaf, those of De11684.2906 and De11684.31669 are the highest in stem, that of De11684.7549 is the highest in root, and those of De11684.7900 are similar in root and leaf and singnificantly (P<0.05) higher than that in stem. bHLH genes in D. elegans have different degrees of responses to different adversity stresses: under 150 mmol·L^-1 NaCl stress, the relative expressions of 7 bHLH genes are all significantly up-regulated at different times; under 250 mmol·L^-1 polyethylene glycol (PEG) stress, the relative expressions of 6 bHLH genes are significantly down-regulated at 2 h; under 4 ℃ stress, the relative expressions of 6 bHLH genes are all significantly upregulated at 8 h. The comprehensive analysis shows that the bHLH transcription factor family members in D. elegans have the basic characteristics of the bHLH transcription factor family; bHLH genes have tissue expression specificity, and bHLH family members may involve in regulation process of salt stress and cold stress.