2024年3月1日 星期五
应用RNA原位杂交技术研究马尾松针叶中银松素合酶的基因表达
Study on pinosylvin synthase(PS)gene expression in needles of Pinus massoniana by RNA in situ hybridization
2006年 第15卷 第3期 页码[12-16]    下载全文[0.9MB]  
摘要

以马尾松(Pinus massoniana Lamb.)银松素合酶(PS)基因为模板,体外转录合成带地高辛标记的反义RNA全长及特异探针,并用这2个探针与马尾松针叶组织石蜡切片进行原位杂交,研究该基因在马尾松针叶中的表达特性以及外界诱导因素对该基因表达的调节特性。结果表明,该基因的转录表达主要集中在针叶的韧皮部;紫外线照射和酵母提取液处理均可使该基因的转录水平明显增加。

Abstract

Using pinosylvin synthase(PS) gene of Pinus massoniana Lamb. as a template, synthesize DIG-labeled antisense RNA full-length and special fragment probes by in vitro transcription with RNA polymerases. To study expression characteristics of PS gene within P. massoniana needles and regulation characteristics of outside induction factors to PS gene expression, two probes prepared were utilized to hybridize with paraffin sections of needles tissue of P. massoniana. The result revealed that PS gene transcripts were predominant in phloem within needles. In addition, the PS gene transcription level was obviously improved by treating with yeast extract and UV radiation.

关键词马尾松; 银松素合酶; RNA; 原位杂交; 石蜡切片
Key wordsPinus massoniana Lamb.; pinosylvin synthase; RNA; in situ hybridization(ISH); paraffin section
作者黄庆煌1,2,叶冰莹1,黄国强2,郭晋隆2,陈由强1,2 ,陈如凯2
所在单位1.福建师范大学生命科学学院,福建福州350007;
2.农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350002
点击量1009
下载次数704
基金项目国家自然科学基金资助项目(30371177); 福建省科学技术厅资助项目(2005N037); 卫生部卫生教育联合攻关计划项目(JA05225/wkj2);