以马尾松(Pinus massoniana Lamb.)银松素合酶(PS)基因为模板,体外转录合成带地高辛标记的反义RNA全长及特异探针,并用这2个探针与马尾松针叶组织石蜡切片进行原位杂交,研究该基因在马尾松针叶中的表达特性以及外界诱导因素对该基因表达的调节特性。结果表明,该基因的转录表达主要集中在针叶的韧皮部;紫外线照射和酵母提取液处理均可使该基因的转录水平明显增加。

Using pinosylvin synthase(PS) gene of Pinus massoniana Lamb. as a template, synthesize DIG-labeled antisense RNA full-length and special fragment probes by in vitro transcription with RNA polymerases. To study expression characteristics of PS gene within P. massoniana needles and regulation characteristics of outside induction factors to PS gene expression, two probes prepared were utilized to hybridize with paraffin sections of needles tissue of P. massoniana. The result revealed that PS gene transcripts were predominant in phloem within needles. In addition, the PS gene transcription level was obviously improved by treating with yeast extract and UV radiation.