2024年7月15日 星期一
基于SSR标记羊角槭子一代和子二代的遗传多样性分析
Analysis on genetic diversity of first filial generation and second filial generation of Acer yangjuechi based on SSR markers
2022年 第31卷 第5期 页码[66-73]    下载全文[2MB]  
摘要

 基于13对SSR引物,对分布于浙江省杭州市西天目山的羊角槭(Acer yangjuechi Fang et P. L. Chiu)子一代及子二代群体共66个样株进行遗传参数和遗传聚类分析。结果显示:13对SSR引物对应位点的观测等位基因数(Na)和有效等位基因数(Ne)分别为6~17和2.4~8.5,均值分别为9.8和4.4;羊角槭子一代群体的Na和Ne值分别为5.7和3.9,分别较子二代群体减少了3.5和1.1。羊角槭子一代群体的观测杂合度略高于子二代群体,子一代群体的等位基因丰富度(3.461)和私有等位基因丰富度(1.648)低于子二代群体。羊角槭66个样株间的遗传相似系数为0.540~0.908。基于遗传相似系数的UPGMA聚类分析结果显示:以遗传相似系数0.709为阈值,羊角槭66个样株分为A、B和C 3组,A组包括51个样株,以遗传相似系数0.723为阈值可进一步分为A1和A2 2个亚组;B组包括14个样株,以遗传相似系数0.718为阈值可进一步分为B1和B2 2个亚组,即Y5、Y22和Y23样株聚为B2亚组,其他11个样株聚为B1亚组;C组仅有Y66样株。综合分析认为,羊角槭群体的遗传多样性水平整体较高,且子二代群体的遗传多样性略高于子一代群体,羊角槭群体具有一定的进化潜力,可采集亲缘关系较远植株的种子进行人工培养,并开展人工杂交授粉,扩大羊角槭的遗传变异,丰富其遗传信息。

Abstract

Genetic parameter and genetic clustering analyses were conducted for 66 sampling individuals of populations of first filial generation and second filial generation of Acer yangjuechi Fang et P. L. Chiu distributed in West Tianmu Mountain of Hangzhou in Zhejiang Province based on 13 pairs of SSR primers. The results show that the number of observed alleles (Na) and number of effective alleles (Ne) of 13 pairs of SSR primers are 6-17 and 2.4-8.5 respectively, and their averages are 9.8 and 4.4 respectively; the Na and Ne values of population of first filial generation of A. yangjuechi are 5.7 and 3.9 respectively, which are 3.5 and 1.1 lower than those of population of second filial generation. The observed heterozygosity of population of first filial generation of A. yangjuechi is slightly higher than that of population of second filial generation, and the allelic richness (3.461) and private allelic richness (1.648) of population of first filial generation are lower than those of population of second filial generation. The genetic similarity coefficient among 66 sampling individuals of A. yangjuechi are 0.540-0.908. The UPGMA clustering analysis result based on genetic similarity coefficient shows that 66 sampling individuals of A. yangjuechi can be divided into three groups namely A, B, and C using the genetic similarity coefficient of 0.709 as the threshold value. Group A contains 51 sampling individuals, and can be further divided into two subgroups namely A1 and A2 using the genetic similarity coefficient of 0.723 as the threshold value; group B contains 14 sampling individuals, and can be further divided into two subgroups namely B1 and B2 using the genetic similarity coefficient of 0.718 as the threshold value, among them Y5, Y22, and Y23 sampling individuals are clustered into subgroup B2, and the other 11 sampling individuals are clustered into subgroup B1; group C only has Y66 sampling individual. Taken together, the overall genetic diversity level of A. yangjuechi population is relatively high, the genetic diversity of population of second filial generation is slightly higher than that of population of first filial generation, and the A. yangjuechi population has some evolutionary potential. It is recommended to collect the seeds of plants with relative far genetic relationships, conduct artificial cultivation, and carry out artificial cross pollination to expand the genetic variation of A. yangjuechi and enrich its genetic information.

关键词羊角槭; 子一代; 子二代; SSR标记; 遗传多样性; 聚类分析
Key wordsAcer yangjuechi Fang et P. L. Chiu; first filial generation; second filial generation; SSR marker; genetic diversity; clustering analysis
作者罗芊芊1,2, 赵明水3, 肖德卿2, 金国庆2, 庞春梅3, 祁祥斌3, 周志春2
所在单位1. 抚州市林业科学研究所, 江西 抚州 344000;2. 中国林业科学研究院亚热带林业研究所 浙江省林木育种技术研究重点实验室, 浙江 杭州 311400;
3. 浙江天目山国家级自然保护区管理局, 浙江 杭州 311300
点击量921
下载次数751
基金项目浙江省珍稀濒危野生动植物物种抢救保护工程天目铁木、羊角槭保护项目(2018—2020)