基于绿豆〔Vigna radiata (Linn.) R. Wilczek〕基因组注释文件鉴定琥珀酸脱氢酶(SDH)基因,并采用生物信息学方法分析了绿豆SDH结构及其编码的蛋白质序列特性；分别运用转录组学和酶学方法分析了镉(Cd)胁迫下绿豆幼苗根、茎和叶中SDH表达量和SDH活性的变化。结果显示:绿豆基因组含有12个SDH基因,分别含0～15个内含子和1～16个外显子。蛋白质序列分析结果显示:VrSDH1.1和VrSDH1.2各含7个motif, VrSDH2含3个motif, VrSDH7.1和VrSDH7.2各含1个motif,其余VrSDH不含motif。VrSDH的相对分子质量为10 789～69 452,理论等电点为pI 4.89至pI 10.96,氨基酸残基数为94～631,亲水性系数为-1.094～0.035,且包含多种结构域。转录组及酶活性分析结果表明:12个VrSDH中VrSDHAF4.2和VrSDH7.2在绿豆幼苗根、茎、叶中不表达,其余10个VrSDH的表达量呈现从根到茎再到叶逐渐递减的趋势；Cd胁迫下,10个VrSDH中多数基因表达量高于对照组,且SDH活性也总体高于对照组。综上,绿豆幼苗中VrSDH参与了Cd胁迫的响应过程。

Succinate dehydrogenase (SDH) genes were identified based on the genome annotation file of Vigna radiata (Linn.) R. Wilczek, and the structures of SDHs of V. radiata and the characteristics of their encoded protein sequences were analyzed by using bioinformatics method; the changes of expression levels of SDHs and SDH activities in roots, stems, and leaves of V. radiata seedlings under cadmium (Cd) stress were analyzed by using transcriptomics and enzymology methods. The results show that there are 12 SDHs in V. radiata genome, which contain 0-15 introns and 1-16 exons. The analysis result of protein sequence reveals that VrSDH1.1 and VrSDH1.2 each contain seven motifs, VrSDH2 contains three motifs, VrSDH7.1 and VrSDH7.2 each contain one motif, and the other VrSDHs lack motifs. The relative molecular masses of VrSDHs are 10 789-69 452, the theoretical isoelectric points are pI 4.89 to pI 10.96, the number of amino acid residues are 94-631, the hydrophilic coefficients are -1.094-0.035, and VrSDHs contain multiple domains. The analysis results of transcriptome and enzyme activities show that among the 12 VrSDHs, VrSDHAF4.2 and VrSDH7.2 are not expressed in the roots, stems, or leaves of V. radiata seedlings, while the expression levels of the other 10 VrSDHs show a decreasing trend from roots to stems and finally to leaves. Under Cd stress, the expression levels of most of the 10 VrSDHs are higher than those in the control group, and the SDH activities are also generally higher than those of the control group. In summary, VrSDHs are involved in the response process of V. radiata seeldings to Cd stress.