2024年12月27日 星期五
深层培养云芝菌丝体蛋白多糖的提取及性质
Extraction and character of proteoglycan from the mycelium of Polystictus versicolor (L. ) Fr. by submerged culture
1998年 第7卷 第4期 页码[19-23]    下载全文[0.6MB]  
摘要

采用水3%草酸和 0 .2 mo l/ L 氢氧化钠为溶剂分别提取深层培养的云芝Polystictus versicolor ( L.) F r.〕菌丝体蛋白多糖, 其得率分别为菌丝体干重的 2.0 %、4.9 %1.9 %。 提取的粗多糖经 Sephadex G-100 柱层析进一步分离纯化, 纯化的多糖经聚丙烯酰胺凝胶电泳分离后呈现一个斑点, Sephadex G-100 柱层析中有一个对称峰, 紫外及红外光谱分析和蛋白质含量测定
表明该多糖为蛋白多糖
, 其蛋白质和多糖含量在水提多糖中分别为 13 %62 %、在酸提多糖中分别为 5%91 %。在碱提多糖中分别为 3%81 %。 组成该多糖的主要单糖是葡萄糖和木糖
 

Abstract

Pro teoglycan were ex tracted by w ater, 3 %oxalic acid and 0 .2 mol/L NaOH from the mycelium of Polystictus versicolor ( L .) Fr .under submerged culture respectively, and ex traction yield of proteog lycan account for mycelium dry w eight was of 2 .0 %( w aterex traction) , 4 .9 % ( acid-extraction) and 1 .9 % ( alkali-extraction) .Proteogly can was further isolated and purified by Sephadex G-100 .Its homogeneity was confirmed by chromatography on Sephadex G-100 and PAGE .By means of I R, UV-spectrum and protein content analy sis that the polysaccharide belongs to proteogly can were determined .Its sugar and protein content were up 62 %and 13 % ( w ater-ex traction) , 91 %and 5 %( acid-extraction) , 81 %and 3 %( alkali-ex traction) respectively .PC and TLC analy sis of proteog ly can hy drolate showed that those sugars were mainly composed of glucose and xylose .
 

关键词云芝; 深层培养; 蛋白多糖; 提取;
Key wordsPolystictus versicolor ( L . ) Fr.; submerged culture; proteoglycan; extraction
作者程光宇, 吴国荣, 邹玉珍, 陈胜兰
所在单位南京师范大学生物系, 南京 210097
点击量1120
下载次数893