摘要 | 采用水、3%草酸和 0 .2 mo l/ L 氢氧化钠为溶剂分别提取深层培养的云芝〔Polystictus versicolor ( L.) F r.〕菌丝体蛋白多糖, 其得率分别为菌丝体干重的 2.0 %、4.9 %和 1.9 %。 提取的粗多糖经 Sephadex G-100 柱层析进一步分离、纯化, 纯化的多糖经聚丙烯酰胺凝胶电泳分离后呈现一个斑点, 在 Sephadex G-100 柱层析中有一个对称峰, 紫外及红外光谱分析和蛋白质含量测定 |
Abstract | Pro teoglycan were ex tracted by w ater, 3 %oxalic acid and 0 .2 mol/L NaOH from the mycelium of Polystictus versicolor ( L .) Fr .under submerged culture respectively, and ex traction yield of proteog lycan account for mycelium dry w eight was of 2 .0 %( w aterex traction) , 4 .9 % ( acid-extraction) and 1 .9 % ( alkali-extraction) .Proteogly can was further isolated and purified by Sephadex G-100 .Its homogeneity was confirmed by chromatography on Sephadex G-100 and PAGE .By means of I R, UV-spectrum and protein content analy sis that the polysaccharide belongs to proteogly can were determined .Its sugar and protein content were up 62 %and 13 % ( w ater-ex traction) , 91 %and 5 %( acid-extraction) , 81 %and 3 %( alkali-ex traction) respectively .PC and TLC analy sis of proteog ly can hy drolate showed that those sugars were mainly composed of glucose and xylose . |
关键词 | 云芝; 深层培养; 蛋白多糖; 提取; |
Key words | Polystictus versicolor ( L . ) Fr.; submerged culture; proteoglycan; extraction |
作者 | 程光宇, 吴国荣, 邹玉珍, 陈胜兰 |
所在单位 | 南京师范大学生物系, 南京 210097 |
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