通过根癌农杆菌 (Agrobacterium tumefaciens)介导将苏云金杆菌 (Bacillus thuringiensis)毒蛋白基因Bt转入杨树NL 80 10 6 (美洲黑杨×小叶杨 ,Populus deltoides×Populus simonii) ,获得了再生植株。PCR及PCR Southernblotting的分析结果表明 ,Bt基因已整合到基因组中。部分转基因植株的杀虫实验表明 ,转基因植株B45和B6 4对一龄舞毒蛾 (Lymantria dispar Linn .)幼虫有明显抗性 ,饲喂转基因杨树叶片的幼虫死亡率显著高于未转基因的对照植株。

Genetically transformed poplar (Populus deltoides × Populus simonii ) plants w ere regenerated after co-cultivation of leaf disks with Agrobacterium tumefaciens strain LBA4404 that harbo red a binary vector pFWZ10 which induced Bacillus thuringiensis δ-endotoxin gene (Bt gene) and npt Ⅱ gene. Some transformations that w ere confirmed by PCR and PCR-Southern blo tting showed obviously insect-resistance compared with the control poplar .Elisa w ill be done to identify the expression of Bt gene in the transformed poplar plants .