对3种百合属（Lilium Linn.）植物鳞茎甲醇提取物中酚类物质含量和抗氧化活性进行比较，分析其中黄酮类及相关化合物的组成，并进行差异代谢物筛选、功能注释和富集分析。结果显示：岷江百合（L. regale Wilson）鳞茎甲醇提取物的DPPH·清除能力和铜离子还原能力显著（P＜0.05）高于龙牙百合（L. brownii var. viridulum Baker）和宝兴百合（L. duchartrei Franch.），而后二者间无显著差异。3种百合属植物中总酚、总黄酮和总黄烷醇的含量与抗氧化活性均呈极显著（P＜0.01）正相关。从3种百合属植物鳞茎甲醇提取物中鉴定出86种黄酮类及相关化合物，其中黄酮类67种（包括花青素4种、黄酮44种、二氢黄酮12种、黄酮醇5种和查尔酮2种）、酚酸类14种、香豆素4种和生物碱1种。与龙牙百合相比，宝兴百合鳞茎甲醇提取物中有34种差异代谢物（18种显著上调，16种显著下调），岷江百合中有43种差异代谢物（38种显著上调，5种显著下调）；与岷江百合相比，宝兴百合中有39种差异代谢物（6种显著上调，33种显著下调）。3种百合属植物中黄酮类及相关化合物主要富集于黄酮、黄酮醇、花青素、酚酸类和苯丙素生物合成通路。总体上看，与龙牙百合相比，岷江百合富集到花青素、黄酮和黄酮醇生物合成通路的黄酮类及相关化合物相对含量显著上调，而在宝兴百合中显著下调或无显著差异；富集到酚酸类和苯丙素生物合成通路的9种黄酮类及相关化合物主要积累在岷江百合中。推测酚酸类、苯丙素、花青素和异鼠李素 O-己糖苷的积累是岷江百合中总黄酮含量高且抗氧化活性强的原因，金圣草黄素 7-O-芸香糖苷、丁香亭 O-己糖苷、柚皮素 7-O-葡萄糖苷和野黑樱素的积累是龙牙百合抗氧化活性较强的原因。综上所述，岷江百合具有较大的开发利用潜力。

Phenolics content and antioxidant activity of methanol extracts from bulbs of three Lilium species were compared， their flavonoids and related compound composition were determined， and screening of differential metabolites， functional annotation， and enrichment analysis were conducted. The results show that DPPH· scavenging ability and reducing capacity of cupric ion of methanol extracts from bulbs of L. regale Wilson are significantly （P<0.05） higher than those of L. brownii var. viridulum Baker and L. duchartrei Franch.， while there is no significant difference between the latter two species. Contents of total phenols， total flavonoids， and total flavanols in three Lilium species all show extremely significant （P<0.01） positive correlations with their antioxidant activities. Eighty-six flavonoids and related compounds are identified from methanol extracts from bulbs of three Lilium species， in which， there are 67 flavonoids （containing 4 anthocyanidins， 44 flavones， 12 flavanones， 5 flavonols， and 2 chalcones）， 14 phenolic acids， 4 coumarins， and 1 alkaloid. Compared with L. brownii var. viridulum， there are 34 differential metabolites （18 of significantly up-regulated and 16 of significantly down-regulated） in methanol extracts from bulbs of L. duchartrei， and 43 differential metabolites （38 of significantly up-regulated and 5 of significantly down-regulated） in L. regale； compared with L. regale， there are 39 differential metabolites （6 of significantly up-regulated and 33 of significantly down-regulated） in methanol extracts from bulbs of L. duchartrei. Flavonoids and related compounds in three Lilium species are mainly enriched in the biosynthesis pathways of flavones， flavonols， anthocyanidins， phenolic acids， and phenylpropanoid. In general， compared with L. brownii var. viridulum， the relative contents of flavonoids and related compounds enriched in the biosynthesis pathways of anthocyanin， flavone， and flavonol are significantly up-regulated in L. regale， while those in L. duchartrei are significantly down-regulated or not significantly regulated. Nine flavonoids and related compounds enriched in the biosynthesis pathway of phenolic acids and phenylpropanoid are mainly accumulated in L. regale. It is speculated that the accumulation of phenolic acids， phenylpropanoid， anthocyanin， and isorhamnetin O-hexoside is responsible for the high content of total flavonoids and strong antioxidant activity in L. regale， while the accumulation of chrysoeriol 7-O-rutinoside, syringetin O-hexoside, naringenin 7-O-glucoside, and prunin is responsible for the strong antioxidant activity in L. brownii var. viridulum. The comprehensive result shows that L. regale has a great potential for development and utilization.