采用超声波辅助浸提-大孔树脂吸附法从中华补血草[ Limonium sinense ( Girard) Kuntze]根、根茎、叶和花中获得多酚提取物,并对它们的得率和组成成分进行了初步分析;在此基础上,比较研究了不同部位多酚提取物对DPPH· 、· OH 和 O2·- 的清除能力。 实验数据表明:中华补血草根、根茎、叶和花多酚提取物的得率分别为 12. 42% 、5. 98% 、 5. 27% 和 3. 98% , 差异明显; 多酚提取物中总酚、 原花色素、 黄烷醇和总黄酮含量变化幅度较大, 分别为51. 87% ~ 61. 60% 、5. 62% ~ 39. 47% 、3. 69% ~ 12. 46% 和 2. 53% ~ 35. 97% ;其中,总酚、原花色素和黄烷醇含量均以根多酚提取物最高,总黄酮含量以花多酚提取物最高,均与其他部位多酚提取物有显著差异。 随质量浓度提高,4 个部位多酚提取物对 3 种自由基的清除率总体上逐渐增大;其中根多酚提取物对 DPPH· 的清除作用最强,半数清除质量浓度(ρ_SC50)为 38. 52 μg· L^-1 , 显著低于阳性对照芦丁和 BHT(ρ_SC50分别为 67. 40 和 74. 25 μg· L^-1 );花多酚提取物对· OH 和 O2·- 的清除能力最强, ρ_SC50分别为 53. 51 和 74. 00 μg· L^-1 ,均低于芦丁;总体上,4 个部位多酚提取物对 DPPH· 的清除能力由强至弱依次为根、根茎、叶、花,对· OH 和 O2·- 的清除能力由强至弱依次为花、根、叶、根茎。 结果显示:中华补血草不同部位多酚提取物均具有较强的自由基清除能力,其中,根和花多酚提取物中高含量的原花色素和黄酮类成分分别与其自由基清除能力密切相关。

By method of ultrasonic wave assisted extraction-macroporous resin adsorption, polyphenol extracts from root, rhizome, leaf and flower of Limonium sinense ( Girard) Kuntze were obtained, and their yield and constituents were preliminarily analyzed. On this basis, scavenging activity of polyphenol extracts from different parts to DPPH· ,· OH and O2·- were comparatively researched. The results show that yield of polyphenol extracts from root, rhizome, leaf and flower is 12. 42% , 5. 98% , 5. 27% and 3. 98% , respectively, having significant difference. Contents of total phenols, proanthocyanidin, flavanol and total flavonoids in polyphenol extracts have a relatively great change range, that is 51. 87% - 61. 60% , 5. 62% -39. 47% , 3. 69% -12. 46% and 2. 53% -35. 97% , respectively. In which, contents of total phenols, proanthocyanidin and flavanol are the highest in polyphenol extracts from root and content of total flavonoids is the highest in polyphenol extracts from flower, showing significant differences with those in other parts. With rising of concentration gradually, scavenging rate of polyphenol extracts from the four parts to three radicals increases generally. In which, the scavenging effect of polyphenol extracts from root to DPPH· is the strongest and its half scavenging concentration (ρ_SC50 ) is 38. 52 μg· L^-1, that is significantly lower than those of positive controls of rutin (67. 40 μg· L^-1 ) and BHT (74. 25 μg· L^-1 ). And scavenging activity of polyphenol extracts from flower to · OH and O2·- is the strongest withρ_SC50 53. 51 and 74. 00 μg · L^-1, respectively, those are lower than that of rutin. Generally, the order of scavenging capacity of polyphenol extracts from the four parts to DPPH· from strong to weak is root, rhizome, leaf, flower, and that to · OH and O2·- from strong to weak is flower, root, leaf, rhizome. Therefore, it could be concluded that polyphenol extracts from different parts of L. sinense have a strong scavenging capacity to radical, in which, high content of proanthocyanidin and flavonoids in polyphenol extracts from root and flower closely relates to radical scavenging activity, respectively.