Effect of NAA and 6-BA with different mass concentrations on primary culture of Helianthus tuberosus ‘Nanyu 9’plantlet was researched, and induction result of different explants was compared. The results show that the primary culture result of shoot tip and stem with axillary bud of H. tuberosus on MS medium without adding NAA and 6-BA is the best, and its germination percentage and normal seedling percentage are the highest (95. 81% and 30. 18% , respectively), and vitrified seedling percentage is the lowest ( only 68. 32% ). Taking tuber, leaf of plantlet, stem without axillary bud and shoot tip and cotyledon of H. tuberosus as explants, callus can be induced on induction medium ( improved MS medium added with 0. 05 mg· L^-1 NAA and 0. 06 mg· L^-1 6-BA, pH 5. 6-pH 5. 8), while only callus from cotyledon can form. adventitious bud and induction rate of adventitious bud is only 14. 3% .