2024年5月27日 星期一
菊芋试管苗的初代培养及愈伤组织不定芽诱导
Primary culture of Helianthus tuberosus plantlet and its adventitious bud induction from callus
2014年 第23卷 第4期 页码[108-110]    下载全文[1.2MB]  
Abstract

Effect of NAA and 6-BA with different mass concentrations on primary culture of Helianthus tuberosus ‘Nanyu 9’plantlet was researched, and induction result of different explants was compared. The results show that the primary culture result of shoot tip and stem with axillary bud of H. tuberosus on MS medium without adding NAA and 6-BA is the best, and its germination percentage and normal seedling percentage are the highest (95. 81% and 30. 18% , respectively), and vitrified seedling percentage is the lowest ( only 68. 32% ). Taking tuber, leaf of plantlet, stem without axillary bud and shoot tip and cotyledon of H. tuberosus as explants, callus can be induced on induction medium ( improved MS medium added with 0. 05 mg· L-1 NAA and 0. 06 mg· L-1 6-BA, pH 5. 6-pH 5. 8), while only callus from cotyledon can form. adventitious bud and induction rate of adventitious bud is only 14. 3% .

关键词菊芋; 试管苗; 外植体; 组织培养; 愈伤组织; 不定芽
Key wordsHelianthus tuberosus Linn.; plantlet; explant; tissue culture; callus; adventitious bud
作者严德凯, 刘兆普, 隆小华
所在单位南京农业大学资源与环境科学学院 江苏省海洋生物学重点实验室, 江苏 南京 210095
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基金项目江苏省农业科技自主创新资金项目[CX(12)1005-1; CX(12)1005-3]; 国家自然科学基金资助项目(31201692); 国家“十二五”科技支撑计划项目(2011BAD13B09); 中央高校基本科研业务费项目(Y0201100249)