2024年10月31日 星期四
太子参快繁技术的优化及同源四倍体的诱导与鉴定
The optimization of rapid propagation technique and the induction and identification of autotetraploid of Pseudostellaria heterophylla
2006年 第15卷 第2期 页码[50-54]    下载全文[0.7MB]  
摘要

The method of rapid propagation in tissue culture of Pseudostellaria heterophylla(Miq.)Pax was optimized by using orthogonal experiment. The autotetraploid induction and identification were carried out in vitro. The results indicated that the best multiplying medium was MS containing 1. 0 mg·L-1 6-BA and 0.2 mg·L-1 NAA. The strong root was induced on the 1/2MS medium containing 0.2 mg·L-1 NAA, 1.0 mg·L-1 ABT and 0. 1 or0. 2 mg·L-1IAA. The autotetraploid strains could be induced by immersing buds in 0. 2% colchicine solution for 22 h or in 0. 3% colchicine solution for12 h. The chromosome number of autotetraploid strain was 2n= 4x= 64.

 

关键词太子参; 快繁; 多倍体; 诱导; 鉴定;
Key wordsPseudostellaria heterophylla(Miq.) Pax; rapid propagation; autotetraploid; inducing; identification
作者谢　燕,高山林,曾　杨,王　蔚,刘　蓁
所在单位中国药科大学,江苏南京210038
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基金项目以太子参〔Pseudostellaria heterophylla(M iq.)Pax〕二倍体组培苗为实验材料,运用正交实验设计和组织培养方法,对太子参试管苗快速繁殖技术进行优化,并进行了同源四倍体的诱导与鉴定。结果表明,太子参最佳的繁殖培养基为含1.0 mg•L-16-BA和0.2 mg•L-1NAA的MS培养基;最佳生根培养基为含0.1 mg•L-1IAA、0.2 mg•L-1NAA和1.0 mg•L-1ABT或0.2 mg•L-1IAA、0.2 mg•L-1NAA和1.0 mg•L-1ABT的1/2MS培养基。诱导同源四倍体的最佳处理方法为:用0.2%秋水仙素处理22 h或用0.3%秋水仙素处理12 h,所诱导的同源泉四倍体染色体数目为2n=4x=64条。