The tissue culture of Pueraria lobata ( Willd .) Ohwi leaf and stem explants are successful for callus induction, and the mature leaf is the best one for forming callus .The combination of NAA 1 .0 mg/ L and BA 3.0 mg/ L as an induced medium is optimum fo r the formation of the calli .The suitable medium for bud formation is MS medium added BA 2.0 mg/ L, AgN O3 4 .24 g/ L and 4-P U 0.5 mg/ L;1.0 mg/ L NAA is effective to induce root ;PP 333 can promote the growth of test-tube plantlets in vitro .