分析了 1/ 2MS 、改良 White 、SJ-1、改良 Nisch、B5和 B5-I 6 种液体培养基、水解乳蛋白(LH)以及 IAA 、IBA 、 NAA 、6-BA 和ABA 5 种激素对西洋参(Panax quinquefolium L .)毛状根生长及皂甙含量的影响。 用高效液相色谱法测定部分毛状根样品的 4 种单体皂甙含量。 结果表明 SJ-1 和 B5培养基较好, 水解乳蛋白可增加毛状根的鲜重, 但降低毛状根中总皂甙的含量。 IAA 、IBA 和 ABA 对毛状根的生长有促进作用, 6-BA 对皂甙积累有显著促进作用, 并较大幅度地提高 Rb1在总甙中所占的比例。
 

The growth speed and saponins content of Panax quinquefolium L.hairy root affected by 6 liquid
media (1/2MS , modified-White , SJ-1 , modified-Nisch , B5 and B5-I), lactalbumin hydrolysace and 5 phytohormones (IAA , IBA , NAA , 6-BA and ABA)were studied.The contents of 4 kinds of ginsenoside have been determined with the method of high performance liquid chromatography. SJ-1 and B5 are fairly good media .The fresh weight increased but the content of saponins decreased when lactalbumin hydrolysace was added .The phytohormones of IAA , IBA and ABA could improve the growth speed of the hairy root , while 6-BA could promote marked the saponin accumulation and raise remarkably the proportion of ginsenoside Rb1 in the total saponin .